Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7427 |
| PDBID: | 6EK2 |
| Chains: | I_B |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.65 |
| Reference: | 10.1016/j.jmb.2018.05.018 |
| Antibody | |
| Antibody: | 10 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Host antigen CD81 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: I
Mutation: NULL
| >6EK2_I|Chain C[auth H], D[auth I]|SINGLE CHAIN FV FRAGMENT|Mus musculus (10090) EVQLVESGGGLVKPGGSLKLSCAASGFTFSDYYMHWVRQTPKKRLEWVATISDGGSYTYFLDSVKGRFTISRDNAKNKLDLQMSSLKSEDTGMYYCARDGNKYSAWFAYWGQGTLVTVSAGGGGSGGGGSGGGGSGGGGSDIQMTQSSSSFSVSLGDRVTITCKASEDIYNRLAWYQQKPGNAPRLLISGATSLETGVPSRFSGSGSGKDYTLSITSLQTEDFATYYCQQYWSPPWTFGGGTKLEIK |
Antigen
Chain: B
Mutation: NULL
| >6EK2_B|Chain A, B|CD81 antigen|Homo sapiens (9606) GSGFVNKDQIAKDVKQFYDQALQQAVVDDDANNAKAVVKTFHETLDCCGSSTLTALTTSVLKNNLCPSGSNIISNLFKEDCHQKIDDLFSGKHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: TYR202 TYR226 TYR228 ASN270 LYS271 TYR272 SER273 ALA274 TYR368 ASN369 ARG370 GLY388 SER405 GLY406 TYR429 TRP430 SER431 PRO432 TRP434 B: GLN125 ASP128 GLN129 GLN132 GLN133 VAL135 VAL136 SER160 THR161 LEU162 THR163 ALA164 LEU165 THR166 THR167 SER168 LEU170 LYS171 ILE181 ASN184 LEU185 PHE186 GLU188 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
