Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7424 |
| PDBID: | 6EJM |
| Chains: | H_B |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.15 |
| Reference: | 10.1016/j.jmb.2018.05.018 |
| Antibody | |
| Antibody: | 5 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Host antigen CD81 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: H
Mutation: NULL
| >6EJM_H|Chain C[auth H], D[auth I]|SINGLE CHAIN FV FRAGMENT|Mus musculus (10090) AEVMLVESGGGFVKPGGSLKLSCAASGFTFRSYIMSWVRQTPEKRLEWVATISGGGGNTYYPDSVKGRFTISRDNAKNTLYLQLSSLRSEDTALYYCASLTAVGDYWGQGTSVTVSSGGGGSGGGGSGGGGSGGGGSDVVMTQTPLTLSVTIGQPASISCKSSQSLFDTDGKTYLTWLLQRPGQSPKRLIYLVSKLASGVPDRFTGSGSGTDFTLKISRVEAEDLGVYYCLQGTHFPLTFGAGTKLDLK |
Antigen
Chain: B
Mutation: NULL
| >6EJM_B|Chain A, B|CD81 antigen|Homo sapiens (9606) GSGFVNKDQIAKDVKQFYDQALQQAVVDDDANNAKAVVKTFHETLDCCGSSTLTALTTSVLKNNLCPSGSNIISNLFKEDCHQKIDDLFSGKLHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR66 ARG68 SER69 ILE71 TRP85 THR88 SER90 GLY91 GLY92 GLY94 ASN95 TYR97 LEU137 THR138 ALA139 VAL140 ASP205 THR206 ASP207 LYS209 TYR211 THR213 ARG225 TYR228 LEU229 LEU268 GLY270 THR271 HIS272 PHE273 LEU275 B: SER160 LEU170 LYS171 ASN173 SER177 GLY178 SER179 ASN180 ILE181 ILE182 SER183 ASN184 LEU185 PHE186 LYS187 GLU188 ASP189 GLN192 LYS193 ASP196 LYS201 LEU202 HIS203 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
