Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7394 |
| PDBID: | 6B20 |
| Chains: | E_BD |
| Organism: | Bos taurus, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.34 |
| Reference: | 10.1038/s41467-018-04432-0 |
| Antibody | |
| Antibody: | Nb5 nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Guanine nucleotide-binding protein Gβγ dimer |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: E
Mutation: NULL
| >6B20_E|Chain E, F|Nanobody against G protein beta gamma dimer|Lama glama (9844) QVQLVESGGGLVQAGGSLRLSCAASGSIFSINAMGWYRQAPGKQRELVAAITRGGRTNYADSVKGRFTLSRDNAKNTVYLQMNSLKPEDTAVYYCNVGRSRGYWGQGTQVTVSS |
Antigen
Chain: BD
Mutation: NULL
| >6B20_B|Chain A, C[auth B]|Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1|Bos taurus (9913) ELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLLSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN >6B20_D|Chain B[auth C], D|Guanine nucleotide-binding protein G(T) subunit gamma-T1|Bos taurus (9913) EDLTEKDKLKMEVDQLKKEVTLERMLVSKCCEEFRDYVEERSGEDPLVKGIPEDKNPFKE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: GLN1 VAL2 GLN3 LEU4 ALA24 SER25 GLY26 ILE28 ILE31 ASN32 ALA33 GLN44 ARG45 THR52 ARG53 ARG56 VAL97 GLY98 ARG99 SER100 ARG101 GLY102 TYR103 TRP104 GLN106 B: ALA56 LYS57 TYR59 GLN75 ASP76 GLY77 LYS78 SER98 TRP99 VAL100 MET101 LEU117 ASP118 ASN119 THR143 GLY144 TYR145 SER147 ASP186 MET188 CYS204 ASP228 ASN230 ASP246 ILE270 THR274 ARG314 SER316 TRP332 D: ASP8 LYS14 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
