Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7388 |
| PDBID: | 7QJJ |
| Chains: | G_E |
| Organism: | Vicugna pacos |
| Method: | XRD |
| Resolution (Å): | 4.60 |
| Reference: | 10.7554/eLife.74589 |
| Antibody | |
| Antibody: | 2 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | NRAMP-related magnesium transporter from the bacterium Eggerthella lenta (EleNRMT) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Chain: G
Mutation: NULL
| >7QJJ_G|Chain C[auth B], F[auth G]|Elen-Nb1-Nb2|Vicugna pacos (30538) QLVESGGGLVLAGGSLRLSCAASVRTFSHYALGWFRQAPGKEREFVAAIRWTGSSANYADSVKGRFTISRDNAKNTVDLRMNSLKPEDTAVYYCAARTVYRPGFEDPNEYAYWGQGTRVTV |
Antigen
Chain: E
Mutation: E88Q/A151S/E193Q/R207H/S244T/I256V/S275A/V366I/V385I/V418L/V429A
| >7QJJ_E|Chain A, D[auth E]|Divalent metal cation transporter|Vicugna pacos (30538) MMMKKNEKLELRDVAVDAAESTELVEVPEKKQPKKIWLLLAALGPGIVTAMAGNDAGGISTYSTVGAKFGFATLWVIPIMCVLLIVVQMTAARMGAVTGKGFAALIRERFGIRLTALAMLALLIGNVATTFSEFAGIASGMEMFGVSKYLSVPVAAVAVWLLVVGGSYKRVEKVFLILSLVFVTYIVAAFMAQPNWEEALTSTVVPHIVNDQSFVSLVIAMIGTTIAPWMMFFNQSNVVEKGVTVKDLFSQKVDVVAGTIAACLVAWFIIVTTGAVLFPQGIEIESAADAARALAPFAGHYAEALFAIGLIAASFLAACVLPLTTAFVICEAFGWEAGVSFKWKEAPLFKSIFTFVIAFSAVVVLIPNIDLMGVMLTAQFVNGLILPVLLVFMAIIAADKRVMGAYRSRIVSRVLIWLTVGIVTVLTAALLVMQVLGI |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| G: HIS31 TYR32 ARG52 TRP53 THR54 SER56 SER57 THR100 VAL101 TYR102 ARG103 PRO104 E: LYS68 PHE69 PHE71 ALA72 GLN193 PRO194 ASN195 GLU198 SER202 HIS207 VAL209 GLY274 ALA275 PHE278 PRO279 GLN280 GLY281 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
