Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7385 |
| PDBID: | 7QJI |
| Chains: | F_E |
| Organism: | Vicugna pacos |
| Method: | XRD |
| Resolution (Å): | 4.10 |
| Reference: | 10.7554/eLife.74589 |
| Antibody | |
| Antibody: | 1 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | NRAMP-related magnesium transporter from the bacterium Eggerthella lenta (EleNRMT) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Chain: F
Mutation: NULL
| >7QJI_F|Chain B[auth C], E[auth F]|Elen-Nanobody-complex|Vicugna pacos (30538) QLQLVESGGGLVQPGGSLRLSCEASGKVFMINAMGWYRQAPGKQRELVAFISRRGNINYADSVKGRFTISRDNAKNTVYLQMNSLRPEDTAIYYCSADPRSNLDDGRYWGKGTPVTV |
Antigen
Chain: E
Mutation: E88Q/A151S/E193Q/R207H/S244T/I256V/S275A/V366I/V385I/V418L/V429A
| >7QJI_E|Chain A, D[auth E]|Divalent metal cation transporter|Vicugna pacos (30538) MMMKKNEKLELRDVAVDAAESTELVEVPEKKQPKKIWLLLAALGPGIVTAMAGNDAGGISTYSTVGAKFGFATLWVIPIMCVLLIVVQMTAARMGAVTGKGFAALIRERFGIRLTALAMLALLIGNVATTFSEFAGIASGMEMFGVSKYLSVPVAAVAVWLLVVGGSYKRVEKVFLILSLVFVTYIVAAFMAQPNWEEALTSTVVPHIVNDQSFVSLVIAMIGTTIAPWMMFFNQSNVVEKGVTVKDLFSQKVDVVAGTIAACLVAWFIIVTTGAVLFPQGIEIESAADAARALAPFAGHYAEALFAIGLIAASFLAACVLPLTTAFVICEAFGWEAGVSFKWKEAPLFKSIFTFVIAFSAVVVLIPNIDLMGVMLTAQFVNGLILPVLLVFMAIIAADKRVMGAYRSRIVSRVLIWLTVGIVTVLTAALLVMQVLGI |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: LYS27 PHE29 MET30 ASN32 SER52 ARG53 ARG54 ASN56 ASN73 ALA74 PRO99 ARG100 SER101 ASN102 ASP104 ARG107 E: GLU142 GLY145 VAL146 SER147 GLN280 GLY281 ILE282 GLU283 ILE284 GLU285 SER286 ALA288 ASP289 ARG292 GLU303 ASN368 ASP370 MET372 GLY373 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
