Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7377 |
| PDBID: | 7PIV |
| Chains: | N_B |
| Organism: | Homo sapiens, synthetic construct, Rattus norvegicus, Mus musculus, Lama glama |
| Method: | EM |
| Resolution (Å): | 2.86 |
| Reference: | 10.1038/s41422-021-00569-8 |
| Antibody | |
| Antibody: | 35 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: N
Mutation: NULL
| >7PIV_N|Chain F[auth N]|Camelid antibody VHH fragment - nanobody 35|Lama glama (9844) QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHH |
Antigen
Chain: B
Mutation: NULL
| >7PIV_B|Chain D[auth B]|Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1|Rattus norvegicus (10116) GPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| N: GLN1 VAL2 GLN3 GLY26 PHE27 THR28 TYR32 ARG98 PRO100 ALA101 PRO102 PHE103 THR114 ALA116 TYR117 GLN120 B: ARG8 GLU12 LYS15 THR184 GLY185 CYS204 ASP205 ALA206 THR223 GLY224 HIS225 GLU226 SER227 ASP228 ASP246 ASP247 ALA248 ILE270 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
