Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7374 |
| PDBID: | 7PIU |
| Chains: | N_A |
| Organism: | Homo sapiens, synthetic construct, Rattus norvegicus, Bos taurus, Lama glama |
| Method: | EM |
| Resolution (Å): | 2.58 |
| Reference: | 10.1038/s41422-021-00569-8 |
| Antibody | |
| Antibody: | 35 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short |
| Antigen mutation: | No |
| Durg Target: | P63092 |
Sequence information
Antibody
Chain: N
Mutation: NULL
| >7PIU_N|Chain F[auth N]|Camelid antibody fragment - nanobody 35|Lama glama (9844) QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >7PIU_A|Chain C[auth A]|Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short|Homo sapiens (9606) MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGDSEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| N: GLY42 LYS43 GLY44 LEU45 GLU46 TRP47 ASP50 SER59 TYR60 THR61 GLY62 SER63 LYS65 PRO100 THR104 ARG105 ASP106 CYS107 PHE108 ASP109 THR111 SER112 THR113 THR114 TYR115 ALA116 TYR117 A: ARG214 ASP215 GLU216 ARG217 ARG218 ILE221 GLN248 THR249 ASN250 GLN253 GLU254 ASN257 LEU258 LYS260 SER261 ILE262 ASN264 ASN265 ARG266 ARG269 ASP296 TYR297 PHE298 PRO299 SER338 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
