Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7366 |
| PDBID: | 7PGB |
| Chains: | UV_W |
| Organism: | Homo sapiens, Alkalilimnicola ehrlichii MLHE-1, Ruegeria pomeroyi DSS-3 |
| Method: | XRD |
| Resolution (Å): | 3.60 |
| Reference: | 10.1038/s41594-022-00775-x |
| Antibody | |
| Antibody: | SAT09 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Voltage-gated ion channel (VGIC) pore domain (PD) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: U
Mutation: NULL
| >7PGB_U|Chain A, AA[auth H], D[auth R], G[auth U], J[auth X], M[auth a], P[auth f], S[auth i], V[auth m], Y[auth p]|SAT09 fab fragment, heavy chain|Homo sapiens (9606) EISEVQLVESGGGLVQPGGSLRLSCAASGFNFSSSSIHWVRQAPGKGLEWVASISSSSGSTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARTYGWYYSWWWAFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: V
Mutation: NULL
| >7PGB_V|Chain B, BA[auth L], E[auth S], H[auth V], K[auth Y], N[auth b], Q[auth g], T[auth k], W[auth n], Z[auth q]|SAT09 fab fragment, light chain|Homo sapiens (9606) SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQGDPRLVTFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: W
Mutation: NULL
| >7PGB_W|Chain C, CA[auth T], DA[auth W], F[auth Z], I[auth c], L[auth d], O[auth e], R[auth h], U[auth l], X[auth o]|Ion transport protein,Voltage-gated sodium channel|Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1) (187272) GPSSPSLLRAIPGIAWIALLLLVIFYVFAVMGTKLFAQSFPEWFGTLGASMYTLFQVMTLESWSMGIARPVIEAYPWAWIYFVSFILVSSFTVLNLFIGIIIESMQSAHHAEDGERTDAYRDEVLARLEQIDQRLNALGETKK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| U: SER31 SER32 SER33 SER50 ILE51 SER52 SER54 SER55 SER57 THR58 SER59 THR99 TRP102 SER105 TRP106 TRP107 TRP108 V: VAL29 SER30 SER31 ALA32 GLY91 ASP92 PRO93 ARG94 W: SER175 PHE176 PRO177 GLU178 TRP179 TYR188 PHE191 GLN192 THR195 GLU197 SER198 TRP199 MET201 GLY202 ILE203 ALA204 ARG205 PRO206 ILE208 GLU209 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
