Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7359 |
| PDBID: | 7PG8 |
| Chains: | NM_Q |
| Organism: | Homo sapiens, Alkalilimnicola ehrlichii MLHE-1, Ruegeria pomeroyi DSS-3 |
| Method: | XRD |
| Resolution (Å): | 4.50 |
| Reference: | 10.1038/s41594-022-00775-x |
| Antibody | |
| Antibody: | ANT05 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Voltage-gated ion channel (VGIC) pore domain (PD) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: N
Mutation: NULL
| >7PG8_N|Chain I[auth B], J[auth F], K[auth I], L, M[auth N], N[auth R], O[auth U], P[auth X]|ANT05 H12 fab fragment, heavy chain|Homo sapiens (9606) EISEVQLVESGGGLVQPGGSLRLSCAASGFNLYSSSIHWVRQAPGKGLEWVASISPSSGSTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARTSRGWISYGSGLDYWGQGTLVTVFNQIKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: M
Mutation: NULL
| >7PG8_M|Chain A, B[auth D], C[auth G], D[auth J], E[auth M], F[auth P], G[auth S], H[auth V]|ANT05 H12 fab fragment, light chain|Homo sapiens (9606) SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQYISYHEPITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: Q
Mutation: NULL
| >7PG8_Q|Chain Q[auth C], R[auth E], S[auth H], T[auth K], U[auth O], V[auth Q], W[auth T], X[auth W]|Ion transport protein,Voltage-gated sodium channel|Alkalilimnicola ehrlichii MLHE-1 (187272) GPSSPSLLRAIPGIAWIALLLLVIFYVFAVMGTKLFAQSFPEWFGTLGASMYTLFQVMTLESWSMGIARPVIEAYPWAWIYFVSFILVSSFTVLNLFIGIIIESMQSAHHAEDGERTDAYRDEVLARLEQIDQRLNALGETKK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| M: SER0 SER28 SER30 ILE92 SER93 TYR94 HIS95 GLU96 PRO97 Q: GLU178 TRP179 LEU196 GLU197 SER198 TRP199 MET201 GLY202 ILE203 ARG205 PRO206 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
