Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7355 |
| PDBID: | 7PAF |
| Chains: | C_A |
| Organism: | synthetic construct, Streptococcus pneumoniae TIGR4 |
| Method: | EM |
| Resolution (Å): | 3.75 |
| Reference: | 10.1126/sciadv.abm1122 |
| Antibody | |
| Antibody: | 7PAF Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Streptococcus pneumoniae choline importer LicB |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >7PAF_C|Chain A[auth B], C|Nanobody|synthetic construct (32630) GSSSQVQLVESGGGSVQAGGSLRLSCAASGTIHAIGYLGWFRQAPGKEREGVAALTTYDGWTYYADSVKGRFTVSLDNAKNTVYLQMNSLKPEDTALYYCAAADDGWMFPLYHNHYEYWGQGTQVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >7PAF_A|Chain B[auth A], D|LicB protein|Streptococcus pneumoniae TIGR4 (170187) MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHDFLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLASSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCALVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQLLGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTIMTSLVVIAGVYIIIKE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: ASP105 GLY106 TRP107 MET108 PHE109 TYR112 ASN114 HIS115 A: PHE118 PHE119 LYS122 LYS124 SER126 LYS127 ASN128 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
