Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7332 |
| PDBID: | 7PA8 |
| Chains: | HG_C |
| Organism: | JC polyomavirus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.15 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | 27C2 Full-length |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | JC polyomavirus major capsid protein VP1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7PA8_H|Chain H[auth HHH], J[auth JJJ], L[auth LLL], N[auth NNN], O[auth YYY]|Fab 27C2 heavy chain|Homo sapiens (9606) EVQLVESGGGLVKPGGSLRLSCAASGFTFSSYTMNWVRQAPGKGLQWVSSISSSSTYMYYGDSVKGRFTISRDNARNSLYLQMNSLRVEDTAVYYCARYAHDWNVDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPV |
Light Chain: G
Mutation: NULL
| >7PA8_G|Chain F[auth FFF], G[auth GGG], I[auth III], K[auth KKK], M[auth MMM]|Fab 27C2 light chain|Homo sapiens (9606) QSVLTQPPSASGTPGQRVTISCSGGSSNIGSNPVNWFQQFPGTAPKLLIYANTQRPSGVPDRFSGSKSGTSVSLAISGLQSEDEGDYHCAAWDDSLKGWVFGGGTKLTVLGAPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKTAASSYLSLTPEQWKSHRSYSCQVTHEGTTVEKTVAP |
Antigen
Chain: C
Mutation: NULL
| >7PA8_C|Chain A[auth AAA], B[auth BBB], C[auth CCC], D[auth DDD], E[auth EEE]|Major capsid protein VP1|JC polyomavirus (10632) GSHMGGVEVLEVKTGVDSITEVECFLTPEMGDPDEHLRGFSKSISISDTFESDSPNRDMLPCYSVARIPLPNLNEDLTCGNILMWEAVTLKTEVIGVTSLMNVHSNGQATHDNGAGKPVQGTSFHFFSVGGEALELQGVLFNYRTKYPDGTIFPKNATVQSQVMNTEHKAYLDKNKAYPVECWVPDPTRNENTRYFGTLTGGENVPPVLHITNTATTVLLDEFGVGPLCKGDNLYLSAVDVCGMFTNRSGSQQWRGLSRYFKVQLRKRRVKN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| G: SER26 GLY30 SER31 ASN32 PRO33 TYR50 ALA51 GLN54 LYS67 GLY69 THR70 ASP94 H: SER31 THR33 SER52 SER53 SER54 THR56 TYR57 HIS101 ASP102 TRP103 C: GLU35 HIS36 LEU37 LYS42 SER43 SER45 ILE46 ASP48 SER54 ASN56 ASN106 GLN108 PHE245 ASN247 ARG248 SER249 SER251 GLN253 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
