Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7301 |
| PDBID: | 7O85 |
| Chains: | NO_M |
| Organism: | Bacillus anthracis, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.30 |
| Reference: | 10.1107/S2059798321007816 |
| Antibody | |
| Antibody: | cAb29 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Protective antigen (PA) |
| Antigen mutation: | No |
| Durg Target: | P13423 |
Sequence information
Antibody
Heavy Chain: N
Mutation: NULL
| >7O85_N|Chain B, E, H, K, N, Q, T|Fab|Mus musculus (10090) AAAAAAAAAAAAAAAAAAAAISCRASQDISNYLNWYAAAAAAAAALLIYYTSRLHSEVPSRFSGSGSGTDYSLTIAAAAAAAAAAAFCQQGKTLPWTFGGGTKL |
Light Chain: O
Mutation: NULL
| >7O85_O|Chain C, F, I, L, O, R, U|Fab|Mus musculus (10090) AAAAAAAAAAAAAAAAAAAAAAKASGYIFTNYNMHWVAAAAAAAAEWIGAIYPRTGDTSYNQKFKGKATLTADKSSSTAYAAAAAAAAAAAAAAACARDGFAYWAAAAAAAAA |
Antigen
Chain: M
Mutation: NULL
| >7O85_M|Chain A, D, G, J, M, P, S|Protective antigen PA-63|Bacillus anthracis (1392) TVPDRDNDGIPDSLEVEGYTVDVKNKRTFLSPWISNIHEKKGLTKYKSSPEKWSTASDPYSDFEKVTGRIDKNVSPEARHPLVAAYPIVHVDMENIILSKNEDQSTQNTDSQTRTISKNTSTSRTHTSEVHGNAEVHASFFDIGGSVSAGFSNSNSSTVAIDHSLSLAGERTWAETMGLNTADTARLNANIRYVNTGTAPIYNVLPTTSLVLGKNQTLATIKAKENQLSQILAPNNYYPSKNLAPIALNAQDDFSSTPITMNYNQFLELEKTKQLRLDTDQVYGNIATYNFENGRVRVDTGSNWSEVLPQIQETTARIIFNGKDLNLVERRIAAVNPSDPLETTKPDMTLKEALKIAFGFNEPNGNLQYQGKDITEFDFNFDQQTSQNIKNQLAELNATNIYTVLDKIKLNAKMNILIRDKRFHYDRNNIAVGADESVVKE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| N: SER30 TYR32 LEU46 TYR49 TYR50 ARG53 HIS55 GLY91 LYS92 THR93 LEU94 TRP96 O: ASN31 TYR32 ASN33 HIS35 TRP47 ALA50 TYR52 ARG54 THR55 ASP57 THR58 SER59 ARG98 ASP99 GLY100 ALA102 M: GLU308 HIS310 ALA311 PHE313 PHE314 ASP315 ILE316 GLY318 ASN539 ASP546 ILE547 THR548 GLU549 GLN560 LYS563 ASN564 ALA567 GLU568 ASN570 ALA571 THR572 ASN573 TYR575 THR576 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
