Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7298 |
| PDBID: | 7NX3 |
| Chains: | ED_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.81 |
| Reference: | 10.1038/s41586-021-03959-5 |
| Antibody | |
| Antibody: | 324 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Anaplastic lymphoma kinase (ALK) |
| Antigen mutation: | No |
| Durg Target: | Q9UM73 |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >7NX3_E|Chain B, E|Fab324 HeavyChain|Mus musculus (10090) QVQLQQSGAELVKPGASVKISCKASGYAFSSYWVNWVKQRPGKGLEWIGQIYPGDGDTNYNGKFKGKATLTADKSSSTAYMQLSSLTSEDSAVYFCARSRGYFYGSTYDSWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGGGTDEVD |
Light Chain: D
Mutation: NULL
| >7NX3_D|Chain C, D|Fab324 Light Chain|Mus musculus (10090) DIVLTQSPASLAVSLGQRATISCRASESVDNYGISFMNWFQQKPGQPPKLLIYAASNQGSGVPARFSGSGSGTDFSLNIHPMEEDDTAMYFCQQSKEVPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >7NX3_A|Chain A, F|ALK tyrosine kinase receptor|Homo sapiens (9606) TAPKSRNLFERNPNKELKPGENSPRQTPIFDPTVHWLFTTCGASGPHGPTQAQCNNAYQNSNLSVEVGSEGPLKGIQIWKVPATDTYSISGYGAAGGKGGKNTMMRSHGVSVLGIFNLEKDDMLYILVGQQGEDACPSTNQLIQKVCIGENNVIEEEIRVNRSVHEWAGGGGGGGGATYVFKMKDGVPVPLIIAAGGGGRAYGAKTDTFHPERLENNSSVLGLNGNSGAAGGGGGWNDNTSLLWAGKSLQEGATGGHSCPQAMKKWGWETRGGFGGGGGGCSSGGGGGGYIGGNAASNNDPEMDGEDGVSFISPLGILYTPALKVMEGHGEVNIKHYLNCSHCEVDECHMDPESHKVICFCDHGTVLAEDGVSCIVSPTPEPHGTDEVD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: TYR32 ILE34 TYR53 ALA54 ASN57 E: ALA28 SER30 SER31 TYR32 TRP33 TYR52 GLY54 ASP55 ASP57 LYS74 GLY101 TYR102 PHE103 TYR104 A: SER785 THR786 ASN787 GLN788 LEU789 ILE790 VAL793 TRP814 LEU890 HIS904 SER905 PRO907 MET910 LYS911 LYS912 TRP913 GLY914 TRP915 GLU916 ARG918 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
