Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7283 |
| PDBID: | 7NK8 |
| Chains: | F_A |
| Organism: | Camelidae mixed library, Influenza A virus (A/Brevig Mission/1/1918(H1N1)), synthetic construct |
| Method: | EM |
| Resolution (Å): | 5.34 |
| Reference: | 10.1038/s41467-021-27950-w |
| Antibody | |
| Antibody: | 8205 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H1N1 polymerase acidic protein |
| Antigen mutation: | No |
| Durg Target: | P03433 |
Sequence information
Antibody
Chain: F
Mutation: NULL
| >7NK8_F|Chain A[auth F]|Nb8205|Camelidae mixed library (1579311) QVQLQESGGGMVQPGGSLRLSCLASGFTFSNYAMTWVRQAPGKGPEWVSMVSNNGADTTYTDSVKGRFTISRDNAKNTLYLRMNNVKPEDSAVYYCAKRRYGGIWTGQPTDYDYLGQGTVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: NULL
| >7NK8_A|Chain B[auth A]|Polymerase acidic protein|Influenza A virus (A/Brevig Mission/1/1918(H1N1)) (88776) MEDFVRQCFNPMIVELAEKAMKEYGEDLKIETNKFAAICTHLEVCFMYSDFHFINERGESIIVESGDPNALLKHRFEIIEGRDRTMAWTVVNSICNTTGAEKPKFLPDLYDYKENRFIEIGVTRREVHIYYLEKANKIKSEKTHIHIFSFTGEEMATKADYTLDEESRARIKTRLFTIRQEMASRGLWDSFRQSERGEETIEERFEITGTMRRLADQSLPPNFSSLENFRAYVDGFEPNGYIEGKLSQMSKEVNARIEPFLKTTPRPLRLPDGPPCSQRSKFLLMDALKLSIEDPSHEGEGIPLYDAIKCMRTFFGWKEPNVVKPHEKGINPNYLLAWKQVLAELQDIENEEKIPKTKNMKKTSQLKWALGENMAPEKVDFDDCKDVSDLKQYDSDEPELRSLASWIQSEFNKACELTDSSWIELDEIGEDVAPIEHIASMRRNYFTAEVSHCRATEYIMKGVYINTALLNASCAAMDDFQLIPMISKCRTKEGRRKTNLYGFIIKGRSHLRNDTDVVNFVSMEFSLTDPRLEPHKWEKYCVLEIGDMLLRSAIGQVSRPMFLYVRTNGTSKIKMKWGMEMRRCLLQSLQQIESMIEAESSVKEKDMTKEFFENKSETWPIGESPKGVEEGSIGKVCRTLLAKSVFNSLYASPQLEGFSAESRKLLLIVQALRDNLEPGTFDLGGLYEAIEECLINDPWVLLNASWFNSFLTHALR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: SER17 SER30 SER52 ASN53 ASN54 ALA56 ASP57 TYR60 GLY66 ARG67 THR69 ARG82 ASN84 ASN85 GLY102 GLY103 A: GLU293 PRO295 HIS297 GLU300 ASP426 GLU427 ILE428 GLU430 ASP431 HIS437 SER440 ARG443 ASN444 ARG490 LYS492 GLU493 GLY494 ARG495 ARG496 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
