Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7281 |
| PDBID: | 7NJ3 |
| Chains: | F_A |
| Organism: | Influenza A virus (A/Brevig Mission/1/1918(H1N1)), Staphylococcus aureus, Camelidae mixed library |
| Method: | EM |
| Resolution (Å): | 4.48 |
| Reference: | 10.1038/s41467-021-27950-w |
| Antibody | |
| Antibody: | 8196 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H1N1 polymerase acidic protein |
| Antigen mutation: | No |
| Durg Target: | P03433 |
Sequence information
Antibody
Chain: F
Mutation: NULL
| >7NJ3_F|Chain F|Nanobody8196 core|Camelidae mixed library (1579311) QVQLQESGGGLVQAGDSLRLSCTTSGSSLSRYGVGWFRQAPGEEREFGASISWNGATYYADSVKGRFTISRDNAKNTVYLQMNSLKAEDTAVYYCAARSDGYLTPYLSPVYWGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: NULL
| >7NJ3_A|Chain A|Polymerase acidic protein|Influenza A virus (strain A/Brevig Mission/1/1918 H1N1) (88776) MEDFVRQCFNPMIVELAEKAMKEYGEDLKIETNKFAAICTHLEVCFMYSDFHFINERGESIIVESGDPNALLKHRFEIIEGRDRTMAWTVVNSICNTTGAEKPKFLPDLYDYKENRFIEIGVTRREVHIYYLEKANKIKSEKTHIHIFSFTGEEMATKADYTLDEESRARIKTRLFTIRQEMASRGLWDSFRQSERGEETIEERFEITGTMRRLADQSLPPNFSSLENFRAYVDGFEPNGYIEGKLSQMSKEVNARIEPFLKTTPRPLRLPDGPPCSQRSKFLLMDALKLSIEDPSHEGEGIPLYDAIKCMRTFFGWKEPNVVKPHEKGINPNYLLAWKQVLAELQDIENEEKIPKTKNMKKTSQLKWALGENMAPEKVDFDDCKDVSDLKQYDSDEPELRSLASWIQSEFNKACELTDSSWIELDEIGEDVAPIEHIASMRRNYFTAEVSHCRATEYIMKGVYINTALLNASCAAMDDFQLIPMISKCRTKEGRRKTNLYGFIIKGRSHLRNDTDVVNFVSMEFSLTDPRLEPHKWEKYCVLEIGDMLLRSAIGQVSRPMFLYVRTNGTSKIKMKWGMEMRRCLLQSLQQIESMIEAESSVKEKDMTKEFFENKSETWPIGESPKGVEEGSIGKVCRTLLAKSVFNSLYASPQLEGFSAESRKLLLIVQALRDNLEPGTFDLGGLYEAIEECLINDPWVLLNASWFNSFLTHALR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: VAL2 TYR32 ARG98 TRP112 A: PRO625 LYS626 SER705 THR712 HIS713 ARG716 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
