Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7280 |
| PDBID: | 7NIR |
| Chains: | F_A |
| Organism: | Influenza A virus (A/Brevig Mission/1/1918(H1N1)), Staphylococcus aureus, Camelidae mixed library |
| Method: | EM |
| Resolution (Å): | 6.70 |
| Reference: | 10.1038/s41467-021-27950-w |
| Antibody | |
| Antibody: | 8191 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H1N1 polymerase acidic protein |
| Antigen mutation: | No |
| Durg Target: | P03433 |
Sequence information
Antibody
Chain: F
Mutation: NULL
| >7NIR_F|Chain F|Nanobody8191 core|Camelidae mixed library (1579311) QVQLQESGGGLVQPGGSLRLSCTASESVASINIVGWYRQISGKERELVARISSGGTITDYSDSVKGRFTITRDNTKNTVYLQMNSLKRGDTAVYYCNAEYQYGSDWFHIWGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: NULL
| >7NIR_A|Chain A|Polymerase acidic protein|Influenza A virus (strain A/Brevig Mission/1/1918 H1N1) (88776) MEDFVRQCFNPMIVELAEKAMKEYGEDLKIETNKFAAICTHLEVCFMYSDFHFINERGESIIVESGDPNALLKHRFEIIEGRDRTMAWTVVNSICNTTGAEKPKFLPDLYDYKENRFIEIGVTRREVHIYYLEKANKIKSEKTHIHIFSFTGEEMATKADYTLDEESRARIKTRLFTIRQEMASRGLWDSFRQSERGEETIEERFEITGTMRRLADQSLPPNFSSLENFRAYVDGFEPNGYIEGKLSQMSKEVNARIEPFLKTTPRPLRLPDGPPCSQRSKFLLMDALKLSIEDPSHEGEGIPLYDAIKCMRTFFGWKEPNVVKPHEKGINPNYLLAWKQVLAELQDIENEEKIPKTKNMKKTSQLKWALGENMAPEKVDFDDCKDVSDLKQYDSDEPELRSLASWIQSEFNKACELTDSSWIELDEIGEDVAPIEHIASMRRNYFTAEVSHCRATEYIMKGVYINTALLNASCAAMDDFQLIPMISKCRTKEGRRKTNLYGFIIKGRSHLRNDTDVVNFVSMEFSLTDPRLEPHKWEKYCVLEIGDMLLRSAIGQVSRPMFLYVRTNGTSKIKMKWGMEMRRCLLQSLQQIESMIEAESSVKEKDMTKEFFENKSETWPIGESPKGVEEGSIGKVCRTLLAKSVFNSLYASPQLEGFSAESRKLLLIVQALRDNLEPGTFDLGGLYEAIEECLINDPWVLLNASWFNSFLTHALR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: TYR37 GLU44 ARG45 LEU47 ARG50 ILE51 ASP59 ASP62 ARG72 ASN74 GLU99 HIS108 ILE109 TRP110 A: PRO275 MET285 ALA404 SER405 SER409 LYS413 THR528 ARG531 LEU532 GLU533 HIS535 ILE554 ARG559 SER624 PRO625 LYS626 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
