Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7278 |
| PDBID: | 7NFT |
| Chains: | C_A |
| Organism: | Influenza A virus (A/duck/Fujian/13/2002(H5N1)), Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.14 |
| Reference: | 10.1038/s41467-021-27950-w |
| Antibody | |
| Antibody: | 8208 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H5N1 polymerase acidic protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >7NFT_C|Chain B[auth C], D|Nb8208|Homo sapiens (9606) QVQLQESGGGLVQPGGSLRLSCAASGRTFSMYAMAWFRQAPGKQREFVGTISRSGDYALHADAVKGRFTISRDNAKNTVYLQMNSLKLEDTAVYYCAAGAYHSKDKTLYDYWGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: NULL
| >7NFT_A|Chain A, C[auth B]|Polymerase basic protein 2|Influenza A virus (A/duck/Fujian/13/2002(H5N1)) (274828) HHHHHHGENLYFQGGGEVRNDDVDQSLIIAARNIVRRATVSTDPLASLLEMCHSTQIGGIRMVDILRQNPTEEQAVDICKAAMGLRISSSFSFGGFTFKRTSGSSVKKEEEVLTGNLQTLKIRVHEGYEEFTMVGRRATAILRKATRRLIQLIVSGRDEQSIAEAIIVAMVFSQEDCMIKAVRGDLNFVNRANQRLNPMHQLLRHFQKDAKVLFQNWGIEPIDNVMGMIGILPDMTPSTEMSLRGVRVSKMGVDEYSSTERVVVSIDRFLRVRDQRGNVLLSPEEVSETQGTERLTITYSSSMM |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: MET31 ALA33 SER52 ARG53 SER54 ASP56 TYR57 ALA58 LEU59 ALA100 TYR101 HIS102 SER103 LYS104 ASP105 LEU108 A: GLU391 GLN447 ILE451 ASN456 ILE461 LEU464 PRO465 ASP466 MET467 THR468 PRO469 SER470 THR471 GLU472 MET473 LYS482 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
