Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7277 |
| PDBID: | 7NFR |
| Chains: | B_A |
| Organism: | Influenza A virus (A/duck/Fujian/13/2002(H5N1)), Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.88 |
| Reference: | 10.1038/s41467-021-27950-w |
| Antibody | |
| Antibody: | 8194 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H5N1 polymerase acidic protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >7NFR_B|Chain B|NB8194|Homo sapiens (9606) QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYYMSWVRQAPGEGREWVSSINRDGSNTYYADSVKGRFTIARDNVKNTLYLLMDSLKSDDTAVYYCTKGSNWPYDNWGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: NULL
| >7NFR_A|Chain A|Polymerase basic protein 2|Influenza A virus (A/duck/Fujian/13/2002(H5N1)) (274828) HHHHHHGENLYFQGGGEVRNDDVDQSLIIAARNIVRRATVSTDPLASLLEMCHSTQIGGIRMVDILRQNPTEEQAVDICKAAMGLRISSSFSFGGFTFKRTSGSSVKKEEEVLTGNLQTLKIRVHEGYEEFTMVGRRATAILRKATRRLIQLIVSGRDEQSIAEAIIVAMVFSQEDCMIKAVRGDLNFVNRANQRLNPMHQLLRHFQKDAKVLFQNWGIEPIDNVMGMIGILPDMTPSTEMSLRGVRVSKMGVDEYSSTERVVVSIDRFLRVRDQRGNVLLSPEEVSETQGTERLTITYSSSMM |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition B: VAL37 GLY44 ARG45 GLU46 TRP47 SER50 TYR59 GLY99 SER100 ASN101 TRP102 PRO103 TYR104 ASP105 A: ARG369 SER387 GLY388 ARG389 ASP390 GLU391 GLN392 ILE394 ILE461 ILE463 MET467 PRO469 VAL480 SER481 LYS482 MET483 GLY484 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
