Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7275 |
| PDBID: | 7NFQ |
| Chains: | C_A |
| Organism: | Influenza A virus (A/duck/Fujian/13/2002(H5N1)), Camelidae mixed library |
| Method: | XRD |
| Resolution (Å): | 1.68 |
| Reference: | 10.1038/s41467-021-27950-w |
| Antibody | |
| Antibody: | 8193 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H5N1 polymerase acidic protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >7NFQ_C|Chain B[auth C], D|Nb8193|Camelidae mixed library (1579311) QVQLQESGGGLVQPGGSLRLSCAASGFTFKMYAMSWVRQAPGKGLEWVSSINSAGGSTSYVDSVKGRFTISRDNAKNTLYLQMNSLKPDDTAVYYCVQGRNWPYDYRGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: NULL
| >7NFQ_A|Chain A, C[auth B]|Polymerase basic protein 2|Influenza A virus (A/duck/Fujian/13/2002(H5N1)) (274828) HHHHHHGENLYFQGGGEVRNDDVDQSLIIAARNIVRRATVSTDPLASLLEMCHSTQIGGIRMVDILRQNPTEEQAVDICKAAMGLRISSSFSFGGFTFKRTSGSSVKKEEEVLTGNLQTLKIRVHEGYEEFTMVGRRATAILRKATRRLIQLIVSGRDEQSIAEAIIVAMVFSQEDCMIKAVRGDLNFVNRANQRLNPMHQLLRHFQKDAKVLFQNWGIEPIDNVMGMIGILPDMTPSTEMSLRGVRVSKMGVDEYSSTERVVVSIDRFLRVRDQRGNVLLSPEEVSETQGTERLTITYSSSMM |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: VAL37 LEU45 GLU46 TRP47 SER50 SER59 VAL61 ASP62 SER63 LYS65 GLY66 ARG67 PHE68 THR69 LYS87 ARG100 ASN101 TRP102 PRO103 TYR104 ASP105 A: GLN257 ARG264 ARG368 ARG369 SER387 GLY388 ARG389 ASP390 GLU391 ILE394 ILE461 ILE463 MET467 PRO469 VAL480 SER481 LYS482 MET483 GLY484 SER519 THR521 GLN522 GLY523 THR524 ARG526 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
