Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7264 |
| PDBID: | 7NA8 |
| Chains: | N_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 2.70 |
| Reference: | 10.1038/s41467-021-26735-5 |
| Antibody | |
| Antibody: | 16 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Guanine nucleotide-binding protein G(i) subunit alpha-1 |
| Antigen mutation: | No |
| Durg Target: | P63096 |
Sequence information
Antibody
Chain: N
Mutation: NULL
| >7NA8_N|Chain D[auth N]|Antibody fragment|Mus musculus (10090) VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSSGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPERFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFGAGTKLEL |
Antigen
Chain: A
Mutation: NULL
| >7NA8_A|Chain A|Guanine nucleotide-binding protein G(i) subunit alpha-1|Homo sapiens (9606) MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATETKNVQFVFDAVTDVIIKNNLKDCGLF |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| N: SER30 SER31 TYR50 SER52 SER53 GLY54 GLY56 THR57 TYR59 ILE100 TYR101 TYR102 TYR103 SER105 HIS167 SER168 ASN169 ASN171 TYR173 TYR175 ARG191 HIS232 LEU233 GLU234 TYR235 A: LEU5 SER6 ALA7 GLU8 ASP9 LYS10 ALA11 ALA12 GLU14 ARG15 MET18 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
