Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7263 |
| PDBID: | 7NA7 |
| Chains: | N_B |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 2.70 |
| Reference: | 10.1038/s41467-021-26735-5 |
| Antibody | |
| Antibody: | 16 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: N
Mutation: NULL
| >7NA7_N|Chain D[auth N]|Antibody fragment|Mus musculus (10090) VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSSGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPERFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFGAGTKLEL |
Antigen
Chain: B
Mutation: NULL
| >7NA7_B|Chain B|Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1|Homo sapiens (9606) MSELDELRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTRQGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPDGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| N: VAL2 GLY26 PHE27 ALA28 SER31 PHE32 ARG98 ILE100 TYR102 TYR103 ASP109 PHE110 ASN169 SER197 B: ASP66 ARG68 LEU69 ASP83 VAL90 HIS91 LEU126 LYS127 ARG129 GLN130 GLY131 ASN132 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
