Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7246 |
| PDBID: | 7N0A |
| Chains: | BA_C |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.10 |
| Reference: | 10.1158/1078-0432.CCR-21-1888 |
| Antibody | |
| Antibody: | MSC1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Falbikitug(Phase-II) |
| Antigen | |
| Antigen: | Leukemia inhibitory factor (LIF) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >7N0A_B|Chain C[auth B]|MSC-1 Fab Heavy chain|Homo sapiens (9606) QVQLQESGGGLVKPGGSLRLSCAASGFTFSHAWMHWVRQAPGKGLEWVGQIKAKSDDYATYYAESVKGRFTISRDDSKNTLYLQMNSLKTEDTAVYYCTCWEWDLDFWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKT |
Light Chain: A
Mutation: NULL
| >7N0A_A|Chain B[auth A]|MSC-1 Fab Light chain|Homo sapiens (9606) DIVMTQTPLSSPVTLGQPASISCRSSQSLLDSDGHTYLNWLQQRPGQPPRLLIYSVSNLESGVPDRFSGSGAGTDFTLKISRVEAEDVGVYYCMQATHAPPYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >7N0A_C|Chain A[auth C]|Leukemia inhibitory factor|Homo sapiens (9606) SPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAFG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: ASP33 HIS35 TYR37 ASN39 LEU51 TYR54 SER55 ASN58 GLU60 SER61 MET94 ALA96 TYR102 B: PHE27 THR28 PHE29 SER30 HIS31 TRP33 LYS52 ALA53 LYS54 SER55 ASP56 TRP101 GLU102 TRP103 ASP104 LEU105 ASP106 C: CYS12 ALA13 ILE14 ARG15 HIS16 PRO17 CYS18 HIS19 ASN20 GLN25 SER28 GLN29 GLN32 ASP120 ILE121 ARG123 GLY124 SER127 ASN128 LEU130 CYS131 CYS134 SER135 HIS138 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
