Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7245 |
| PDBID: | 7MXE |
| Chains: | RW_J |
| Organism: | Homo sapiens, Human immunodeficiency virus 1, Macaca mulatta |
| Method: | EM |
| Resolution (Å): | 3.70 |
| Reference: | 10.1038/s41541-021-00387-4 |
| Antibody | |
| Antibody: | Ab1245 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 BG505 SOSIP gp120 |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: R
Mutation: NULL
| >7MXE_R|Chain M[auth R]|Ab1245 Fab heavy chain|Macaca mulatta (9544) MGWSCIILFLVATATGVHSQVQLQQSGPGLVKPSETLSLTCTVSGGSISESYYWKWIRQPPGKGLEWVGRIYGSGVSTSYNPSLESRVTISKDTSKNHFSLKLTSVTAADTAVYYCARSYVRAETLMFASLGGPYNRFDVWGAGVLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSXALTSGVHTFPAVLQSSXLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHHHHHH |
Light Chain: W
Mutation: NULL
| >7MXE_W|Chain N[auth W]|Ab1245 Fab light chain|Macaca mulatta (9544) MGWSCIILFLVATATGSWAQSALTQPSSASASLGASVTLTCTLSSGYSNSAVDWHQQRPGKGPQFVMRVGTGGIVGSKGDGIPDRFSGSGSGLNRYLIIKNIQEEDESDYHCGADHGTGSAFVYVFGGGTKLTVLGQPKASPLVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: J
Mutation: T299N/A467C/E475R/K476R
| >7MXE_J|Chain F[auth G], H[auth J], L[auth P]|Envelope glycoprotein BG505 SOSIP.664 gp120|Human immunodeficiency virus 1 (11676) NLWVTVYYGVPVWKDAETTLFCASDAKAYETEKHNVWATHACVPTDPNPQEIHLENVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLQCTNVTNNITDDMRGELKNCSFNMTTELRDKKQKVYSLFYRLDVVQINENQGNRSNNSNKEYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCPSVSTVQCTHGIKPVVSTQLLLNGSLAEEEVMIRSENITNNAKNILVQFNTPVQINCTRPNNNTRKSIRIGPGQAFYATGDIIGDIRQAHCNVSKATWNETLGKVVKQLRKHFGNNTIIRFANSSGGDLEVTTHSFNCGGEFFYCNTSGLFNSTWISNTSVQGSNSTGSNDSITLPCRIKQIINMWQRIGQAMYAPPIQGVIRCVSNITGLILTRDGGSTNSTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRCKRRVVGRRRRRR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| R: GLY26 SER28 GLU31 SER32 TYR33 SER53 GLY54 VAL55 SER56 TYR96 VAL97 ARG98 ALA99 J: TYR39 TYR40 GLY41 VAL42 PRO43 TRP45 PRO79 ASN80 PRO81 GLN82 GLU83 ILE84 HIS85 LEU86 GLU87 ASN88 VAL89 LYS227 LYS229 LYS231 GLU267 VAL496 ALA497 PRO498 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
