Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7243 |
| PDBID: | 7MXE |
| Chains: | AB_J |
| Organism: | Homo sapiens, Human immunodeficiency virus 1, Macaca mulatta |
| Method: | EM |
| Resolution (Å): | 3.70 |
| Reference: | 10.1038/s41541-021-00387-4 |
| Antibody | |
| Antibody: | 8ANC195 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 BG505 SOSIP gp120 |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >7MXE_A|Chain A, G[auth H], J[auth M]|8ANC195 G52K5 Fab heavy chain|Homo sapiens (9606) QIHLVQSGTEVKKPGSSVTVSCKAYGVNTFGLYAVNWVRQAPGQSLEYIGQIWRWKSSASHHFRGRVLISAVDLTGSSPPISSLEIKNLTSDDTAVYFCTTTSTYDRWSGLHHDGVMAFSSWGQGTLISVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHHHHHH |
Light Chain: B
Mutation: NULL
| >7MXE_B|Chain B, I[auth L], K[auth O]|8ANC195 G52K5 Fab light chain|Homo sapiens (9606) DIQMTQSPSTLSASTGDTVRISCRASQSITGNWVAWYQQRPGKAPRLLIYRGAALLGGVPSRFRGSAAGTDFTLTIGNLQAEDFGTFYCQQYDTYPGTFGQGTKVEVKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: J
Mutation: T299N/A467C/E475R/K476R
| >7MXE_J|Chain F[auth G], H[auth J], L[auth P]|Envelope glycoprotein BG505 SOSIP.664 gp120|Human immunodeficiency virus 1 (11676) NLWVTVYYGVPVWKDAETTLFCASDAKAYETEKHNVWATHACVPTDPNPQEIHLENVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLQCTNVTNNITDDMRGELKNCSFNMTTELRDKKQKVYSLFYRLDVVQINENQGNRSNNSNKEYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCPSVSTVQCTHGIKPVVSTQLLLNGSLAEEEVMIRSENITNNAKNILVQFNTPVQINCTRPNNNTRKSIRIGPGQAFYATGDIIGDIRQAHCNVSKATWNETLGKVVKQLRKHFGNNTIIRFANSSGGDLEVTTHSFNCGGEFFYCNTSGLFNSTWISNTSVQGSNSTGSNDSITLPCRIKQIINMWQRIGQAMYAPPIQGVIRCVSNITGLILTRDGGSTNSTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRCKRRVVGRRRRRR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: ASN28 THR29 GLY31 LEU32 ARG54 TRP55 GLY72 SER73 SER74 THR97 TYR98 ASP99 J: VAL44 TRP45 LYS46 ASP47 VAL89 THR90 GLU91 GLU92 PHE93 ASN94 LYS97 THR236 GLY237 PRO238 GLU275 ASN276 ILE277 THR278 LYS351 HIS352 PHE353 ARG456 THR461 LYS487 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
