Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 723 |
| PDBID: | 4FHB |
| Chains: | D_A |
| Organism: | Escherichia coli K-12, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.80 |
| Reference: | 10.1016/j.bbapap.2013.07.010 |
| Antibody | |
| Antibody: | Nb179 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Dihydrofolate reductase |
| Antigen mutation: | No |
| Durg Target: | P0ABQ4 |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >4FHB_D|Chain B[auth D]|Nb179|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCEASGRTFSSYAMGWFRQAPGKERDFVAVISWSGSNTYYADSAKGRFTISRDNAKNTVYLQMNSLKPEDTAIYYCAAPGRPHGSSWSLNKKGQGYDYWGQGTQVTVSSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >4FHB_A|Chain A|Dihydrofolate reductase|Escherichia coli (83333) MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: TYR32 SER52 TRP53 SER54 SER56 ASN57 THR58 GLY100 ARG101 PRO102 HIS103 GLY104 SER105 TRP107 SER108 LYS111 A: ALA9 VAL10 ASP11 ARG12 HIS114 ILE115 ASP116 GLU118 ASP127 TYR128 GLU129 PRO130 PHE140 SER150 CYS152 GLU154 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
