Details

Antibody

Heavy Chain: G
Mutation: NULL

>7MLY_G|Chain B[auth F], D[auth G], F[auth H], H[auth L]|3D1 Fab Heavy Chain|Rattus norvegicus (10116) QVQLQQSGAELMKPGAAVKISCKATGHTISRYWIDWLKQRPGHGLEWIGEILPGSGSTNYNEKFKGKATFTAEKSSNTAYMQLSSLTSEDSAVYYCAMGVRGNYFDYWGQGTTLTVSS

Light Chain: J
Mutation: NULL

>7MLY_J|Chain A[auth I], C[auth J], E[auth K], G[auth M]|3D1 Fab Light Chain|Rattus norvegicus (10116) DIVMTQSHKFMSTSVGDRVSITCKASQDVSTAVAWYQQKPGQSPKLLIYWASTRHTGVPGRFTGSGSGTDYTLTISSVQAEDLSLYYCQQHYSTPRTFGGGTKLEIK

Antigen

Chain: B
Mutation: NULL

>7MLY_B|Chain I[auth A], J[auth B], K[auth C], L[auth D]|Glycine receptor alpha 1|Sus scrofa (9823) MYRFNTLRLYLWETIVFFSLAASKEAEAARSASKPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVSCNIFINSFGSIAETTMDYRVNIFLRQQWNDPRLAYNEYPDDSLDLDPSMLDSIWKPDLFFANEKGAHFHEITTDNKLLRISRNGNVLYSIRITLTLACPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQEQGAVQVADGLTLPQFILKEEKDLRYCTKHYNTGKFTCIEARFHLERQMGYYLIQMYIPSLLIVILSWISFWINMDAAPARVGLGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRRHHKSPMLNLFQEDEAGEGRFNFSAYGMGPACLQAKDGISVKGANNTTTNPPPAPSKSPEEMRKLFIQRAKKIDKISRIGFPMAFLIFNMFYWIIYKI

Interaction

1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Ų were classified as interacting residues.

Interacting residues (ΔSASA based)

J: ASP28 VAL29 SER30 THR31 TRP50 HIS91 TYR92 ARG96 G: SER30 ARG31 TYR32 TRP33 GLU50 LEU52 SER55 SER57 GLY99 VAL100 ARG101 GLY102 ASN103 B: LYS61 GLY62 PRO63 PRO64 MET191 ASN192 ILE195 GLU197 TYR225 THR227 HIS229 TYR230 ASN231 GLY233 LYS234

Interacting residues (Atom distance based)

Within the threshold, the structure has no interacting amino acids and cannot be displayed. This may be due to the following reasons:
1. The method used to determine the structure is electron microscopy or nuclear magnetic resonance, which results in too low resolution;
2. the coordinates of some amino acids involved in the interaction in the structure were not detected due to technical limitations. The results are less based on atomic coordinates, but the antibody does target the antigen.