Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7224 |
| PDBID: | 7MJT |
| Chains: | AB_C |
| Organism: | synthetic construct, Streptomyces lividans |
| Method: | XRD |
| Resolution (Å): | 3.30 |
| Reference: | 10.1038/s41467-022-28866-9 |
| Antibody | |
| Antibody: | 7MUB Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | pH-gated potassium channel KcsA |
| Antigen mutation: | Yes |
| Durg Target: | P0A333; P0A334; |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >7MJT_A|Chain A|Fab heavy chain|synthetic construct (32630) EISEVQLVESGGGLVQPGGSLRLSCAASGYTFTSDWIHWVRQAPGKGLEWIGEIIPSYGRANYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARERGDGYFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: B
Mutation: NULL
| >7MJT_B|Chain B|Fab light chain|synthetic construct (32630) SDIQMTQSPSSLSASVGDRVTITCRASQSIGTDIHWYQQKPGKAPKLLIKYASESISGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQSNRWPFTFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: A3C/E46V/L65C/R92Q/E93C/E95Q/R96Q
| >7MJT_C|Chain C|pH-gated potassium channel KcsA|Streptomyces lividans (1916) WRCAGAATVLLVIVLLAGSYLAVLAERGAPGAQLITYPRALWWSVVTATTVGYGDLYPVTLWGRCVAVVVMVAGITSFGLVTAALATWFVGQCQQQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: THR33 SER34 TRP36 GLU53 ILE55 TYR58 ARG60 ASN62 GLU102 ARG103 GLY104 ASP105 GLY106 B: ASP2 ASP33 TYR51 SER92 ASN93 ARG94 TRP95 PRO96 PHE97 C: TYR45 LEU46 VAL48 LEU49 ARG52 GLY53 ALA54 PRO55 GLY56 ALA57 GLN58 ILE60 THR61 TYR62 PRO63 ARG64 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
