Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 722 |
| PDBID: | 4F9P |
| Chains: | D_B |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.52 |
| Reference: | 10.1074/jbc.M112.384354 |
| Antibody | |
| Antibody: | 103.2 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Butyrophilin subfamily 3 member A1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >4F9P_D|Chain C, D|103.2 anti-BTN3A1 antibody fragment|Mus musculus (10090) QVQLQQSGAEVVRPGTSVKVSCKASGYAFTSYLIHWIKQRPGQGLEWIGVINPRSGDSHYNEKFKDRTTLTADQSSSTAYMQLSSLTSDDSAVYFCARSDYGAYWGQGTLVTVSAAGGGGSGGGGSGGGGSGGGGSDIVLTQSPVTLSVTPGDSVSLSCRASQSISNNLHWYRQKSHESPRLLIKYASQSIFGIPSRFSGSGSGTEFTLSINSVETEDFGIYFCQQSNSWPHTFGTGTKLELKRADAAAASGAD |
Antigen
Chain: B
Mutation: NULL
| >4F9P_B|Chain A, B|Butyrophilin subfamily 3 member A1|Homo sapiens (9606) ADLQFSVLGPSGPILAMVGEDADLPCHLFPTMSAETMELKWVSSSLRQVVNVYADGKEVEDRQSAPYRGRTSILRDGITAGKAALRIHNVTASDSGKYLCYFQDGDFYEKALVELKVAALGSDLHVDVKGYKDGGIHLECRSTGWYPQPQIQWSNNKGENIPTVEAPVVADGVGLYAVAASVIMRGSSGEGVSCTIRSSLLGLEKTASISIADPFFRSAQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: THR30 SER31 TYR32 LEU33 ASN52 PRO53 ARG54 SER55 ASP57 HIS59 SER99 ASP100 TYR101 ASN167 ASN168 TYR186 SER227 ASN228 TRP230 B: SER30 GLU32 THR33 ALA51 ASP52 GLY53 LYS54 ILE70 LEU71 ARG72 ASP73 ILE75 THR76 ASP101 GLY102 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
