Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 721 |
| PDBID: | 4F9L |
| Chains: | C_B |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.14 |
| Reference: | 10.1074/jbc.M112.384354 |
| Antibody | |
| Antibody: | 20.1 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Butyrophilin subfamily 3 member A1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >4F9L_C|Chain B[auth C], C[auth D]|20.1 anti-BTN3A1 antibody fragment|Mus musculus (10090) DIVLTQSPSSLSASLGDTITITCHASQNINLWLSWYQQRPGNIPKLLIYRASNLHTGVPSRFSGSGSATGFTLTISSLQPEDIATYYCQQGHSYPYTFGGGTKLDIKRADAGGGGSGGGGSGGGGSGGGGSQVQLQESGAELVKPGASVKLSCKASGYTFTRYYLYWVKQRPGQGLEWIGEINPNNGGTKFNEKFKSKATLTVDKSSRTTYIQLSSLTSEDSAVYYCSREDDYDGTPDAMDYWGQGTAVTVSSAASGAD |
Antigen
Chain: B
Mutation: NULL
| >4F9L_B|Chain A, D[auth B]|Butyrophilin subfamily 3 member A1|Homo sapiens (9606) ADLQFSVLGPSGPILAMVGEDADLPCHLFPTMSAETMELKWVSSSLRQVVNVYADGKEVEDRQSAPYRGRTSILRDGITAGKAALRIHNVTASDSGKYLCYFQDGDFYEKALVELKVAALGSDLHVDVKGYKDGGIHLECRSTGWYPQPQIQWSNNKGENIPTVEAPVVADGVGLYAVAASVIMRGSSGEGVSCTIRSSLLGLEKTASISIADPFFRSAQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: TRP32 GLY91 HIS92 SER93 TYR94 THR159 THR161 ARG162 TYR163 TYR164 GLU181 ASN183 ASN185 ASN186 LYS190 LYS205 GLU230 ASP234 GLY235 THR236 PRO237 B: LEU43 ARG44 GLN45 VAL46 GLU57 ASP58 ARG59 GLN60 SER61 ALA62 PRO63 ARG65 ARG67 GLY169 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
