Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7209 |
| PDBID: | 7MEJ |
| Chains: | A_R |
| Organism: | Lama glama, Severe acute respiratory syndrome coronavirus 2 |
| Method: | EM |
| Resolution (Å): | 3.55 |
| Reference: | 10.1038/s41467-021-24963-3 |
| Antibody | |
| Antibody: | 21 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV-2 spike glycoprotein RBD |
| Antigen mutation: | No |
| Durg Target: | P0DTC2; P0DTC2; |
Sequence information
Antibody
Chain: A
Mutation: NULL
| >7MEJ_A|Chain A|Nanobody Nb21|Lama glama (9844) QVQLVESGGGLVQAGGSLRLSCAVSGLGAHRVGWFRRAPGKEREFVAAIGANGGNTNYLDSVKGRFTISRDNAKNTIYLQMNSLKPQDTAVYYCAARDIETAEYTYWGQGTQVTVSS |
Antigen
Chain: R
Mutation: NULL
| >7MEJ_R|Chain B[auth R]|Spike protein S1|Severe acute respiratory syndrome coronavirus 2 (2697049) TNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: GLY28 ALA29 HIS30 ARG31 PHE35 ARG43 GLU44 PHE45 ALA51 ASN52 ASN55 ASN57 ASN72 ARG97 ILE99 GLU100 R: GLY446 TYR449 ASN450 LEU452 LEU455 THR470 GLY482 VAL483 GLU484 TYR489 PHE490 LEU492 GLN493 SER494 TYR495 GLY496 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
