Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7191 |
| PDBID: | 5T5F |
| Chains: | HL_A |
| Organism: | Neisseria meningitidis, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.98 |
| Reference: | 10.1042/BCJ20160806 |
| Antibody | |
| Antibody: | JAR5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Factor H-binding protein (fHbp) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5T5F_H|Chain B[auth H]|Monoclonal antibody Jar5 heavy chain|Mus musculus (10090) QVQMQQPGAELVKPGASVKLSCKASGYTFISYWMHWVKQRPGRGLEWIGRIAPDTGIIYYNEKFKNKATLTVDTPSSTAYMQLNSLTSEDSAVYYCARYLKYDGSTYRFDYWGQGTTLTVSSAKTTPPSVYPLAPGSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSPWPSETVTCNVAHPASSTKVDKK |
Light Chain: L
Mutation: NULL
| >5T5F_L|Chain C[auth L]|Monoclonal antibody Jar5 light chain|Mus musculus (10090) DIVMTQAAPSVPVTPGESVSISCRSSKSLLHSNGNTYLFWFLQRPGQSPQLLIYRMSNLASGVPDRFSGSGSGTSFTLRISRVEAEDVGVYYCMQHLEYPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNR |
Antigen
Chain: A
Mutation: NULL
| >5T5F_A|Chain A|Factor H binding protein variant B24|Neisseria meningitidis (487) GGGGVAADIGAGLADALTAPLDHKDKGLQSLTLDQSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGEFQVYKQSHSALTAFQTEQIQDSEHSGKMVAKRQFRIGDIAGEHTSFDKLPEGGRATYRGTAFGSDDAGGKLTYTIDFAAKQGNGKIEHLKSPELNVDLAAADIKPDGKRHAVISGSVLYNQAEKGSYSLGIFGGKAQEVAGSAEVKTVNGIRHIGLAAKQL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLY26 TYR27 THR28 SER31 TYR32 TRP33 ARG50 ALA52 ASP54 THR55 ILE57 TYR59 TYR99 LEU100 LYS101 TYR102 ASP103 GLY104 SER105 THR106 TYR107 ARG108 L: HIS31 SER32 ASN33 ASN35 TYR37 HIS96 LEU97 TYR99 TYR101 A: ILE82 VAL84 ASP85 GLY86 GLN87 ILE89 THR90 LEU91 GLN113 GLN115 SER117 GLU118 HIS119 SER120 GLY121 LYS122 MET123 VAL124 ALA125 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
