Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7190 |
| PDBID: | 5T1D |
| Chains: | HL_B |
| Organism: | Human herpesvirus 4 strain B95-8, human gammaherpesvirus 4, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.10 |
| Reference: | 10.1038/ncomms13557 |
| Antibody | |
| Antibody: | E1D1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human herpesvirus 4 envelope glycoprotein L |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5T1D_H|Chain D[auth H]|E1D1 IgG2a heavy chain|Mus musculus (10090) EVQLEQSGPELVKPGASVKMSCKASGYTFTDYVISWVKQRTGQGLEWIGEIYPESGNTYYNEKFKGEATLTADKSSNTAYMQLSRLTSEDSAVYFCAEGYAMDFWGQGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIV |
Light Chain: L
Mutation: NULL
| >5T1D_L|Chain E[auth L]|E1D1 IgG2a light chain|Mus musculus (10090) DIVITQTPLSLPVSLGDQASISCRSSQSLLHSNGNTYLHWYLQRPGQSPKLLIYKVSNRFSGVPDRFSGSGSGTDFTLMINRVEAEDLGVYFCSQSIHVPRTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRN |
Antigen
Chain: B
Mutation: NULL
| >5T1D_B|Chain B|Envelope glycoprotein L|Epstein-Barr virus (strain B95-8) (10377) WAYPCCHVTQLRAQHLLALENISDIYLVSNQTCDGFSLASLNSPKNGSNQLVISRCANGLNVVSFFISILKRSSSALTGHLRELLTTLETLYGSFSVEDLFGANLNRYAWHR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLU1 VAL2 LYS23 ALA24 SER25 GLY26 TYR27 THR28 PHE29 THR30 ASP31 TYR32 GLU50 TYR52 GLU54 SER55 ASN57 TYR59 SER76 ASN77 TYR100 ALA101 ASP103 L: HIS31 SER32 ASN33 GLY34 ASN35 TYR37 HIS39 TYR54 LYS55 SER96 ILE97 VAL99 B: PRO27 CYS28 CYS29 SER63 ASN72 GLN73 LEU74 VAL75 ILE76 SER77 ARG78 CYS79 ASN127 ASN129 ARG130 TYR131 ALA132 TRP133 HIS134 ARG135 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
