Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7184 |
| PDBID: | 5OJM |
| Chains: | O_E |
| Organism: | synthetic construct, Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.30 |
| Reference: | 10.1038/nsmb.3484 |
| Antibody | |
| Antibody: | 25 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Chimaeric beta3-alpha5 type A γ-aminobutyric acid receptor (GABAA R) |
| Antigen mutation: | No |
| Durg Target: | P31644 |
Sequence information
Antibody
Chain: O
Mutation: NULL
| >5OJM_O|Chain F[auth K], G[auth L], H[auth M], I[auth N], J[auth O]|Nanobody Nb25|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCAASGHTFNYPIMGWFRQAPGKEREFVGAISWSGGSTSYADSVKDRFTISRDNAKNTVYLEMNNLKPEDTAVYYCAAKGRYSGGLYYPTNYDYWGQGTQVTVSS |
Antigen
Chain: E
Mutation: NULL
| >5OJM_E|Chain A, B, C, D, E|Human GABAA receptor chimera beta3-alpha5,Gamma-aminobutyric acid receptor subunit beta-3,Gamma-aminobutyric acid receptor subunit alpha-5|synthetic construct (32630) MGILPSPGMPALLSLVSLLSVLLMGCVAETGQSVNDPGNMSFVKETVDKLLKGYDIRLRPDFGGPPVCVGMNIDIASIDMVSEVNMDYTLTMYFQQYWRDKRLAYSGIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGGDKAVTGVERIELPQFSIVEHRLVSRNVVFATGAYPRLSLSFRLKRNIGYFVIQTYLPCIMTVILSQVSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKSQPARAAKIDKMSRIVFPILFGTFNLVYWATYLNREPVIKGATSPKGTTETSQVAPA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| O: THR28 PHE29 ASN30 TRP53 ARG101 TYR102 SER103 GLY104 GLY105 TYR108 THR110 ASN111 ASP113 E: LEU99 ASN100 ALA135 MET137 MET138 ASP139 ARG141 ASN149 THR151 GLU153 ARG196 ASN197 VAL198 VAL199 PHE200 ALA201 ARG207 TYR285 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
