Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7181 |
| PDBID: | 5OCC |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1016/j.bpj.2018.03.040 |
| Antibody | |
| Antibody: | 6G08 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | CD32b (Fc gamma receptor IIb) |
| Antigen mutation: | No |
| Durg Target: | P31995; P31994; P12318; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5OCC_H|Chain B[auth H]|6G08 Fab heavy chain|Homo sapiens (9606) EVQLLESGGGLVQPGGSLRLSCAASGFTLSSYGISWVRQAPGKGLEWVSGISGSGGNTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCASSVGAYANDAFDIWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: L
Mutation: NULL
| >5OCC_L|Chain C[auth L]|6G08 Fab Light Chain|Homo sapiens (9606) QSVLTQPPSASGTPGQRVTISCTGSSSNIGAGYDVHWYQQLPGTAPKLLIYGDTNRPSGVPDRFSGSKSGTSASLAISGLRSEDEADYYCAAWDDSLNGPVFGGGTKLTVLGQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: NULL
| >5OCC_A|Chain A|Low affinity immunoglobulin gamma Fc region receptor II-b|Homo sapiens (9606) TPAAPPKAVLKLEPQWINVLQEDSVTLTCRGTHSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIVLRCHSWKDKPLVKVTFFQNGKSKKFSRSDPNFSIPQANHSHSGDYHCTGNIGYTLYSSKPVTITVQAPS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER31 SER52 GLY53 SER54 GLY55 GLY56 ASN57 THR58 TYR59 ASN74 ALA102 TYR103 ALA104 ASN105 ASP106 L: ILE29 GLY30 ALA31 GLY32 TYR33 ASP34 TYR51 GLY52 ASN55 LYS68 GLY70 TRP93 ASP95 ASN98 A: GLU55 PRO56 TRP58 GLU64 ASP65 SER66 LYS102 ARG151 HIS153 LYS156 ASP157 LYS158 PRO159 LEU160 VAL161 ARG176 SER177 ASP178 PRO179 ASN180 ILE200 GLY201 TYR202 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
