Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 718 |
| PDBID: | 4F2M |
| Chains: | AB_E |
| Organism: | Mus musculus, TGEV virulent Purdue |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1371/journal.ppat.1002859 |
| Antibody | |
| Antibody: | 1AF10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | TGEV coronavirus Spike protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >4F2M_A|Chain A, C|monoclonal antibody 1AF10, heavy chain|Mus musculus (10090) QVQLQQSGPELVKPGASVKISCKASGYAFSSSWMNWVKQRPGQGLEWIGRIYPGDGETNYSEKFKGKATLTADKSSSTAYMHLSSLTSVDSAVYFCARGGYRYDPYYAMDYWGQGTSVTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEP |
Light Chain: B
Mutation: NULL
| >4F2M_B|Chain B, D|monoclonal antibody 1AF10, light chain|Mus musculus (10090) DILLTQSPAILSVSPGERVSLSCRASQSIGTSIHWYQQRTNGSPRPLIKYASESISGIPSRFSGSGSGTDFTLNINSVESEDIADYFCQQTDSWPTTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: E
Mutation: NULL
| >4F2M_E|Chain E, F|Spike protein|TGEV virulent Purdue (398812) YPYDVPDYAGAQPARSPGLVPRGSRTANLNNGFYPVSSSEVGLVNKSVVLLPSFYTHTIVNITIDLGMKRSGYGQPIASTLSNITLPMQDNNTDVYCIRSDQFSVYVHSTCKSSLWDNIFKRNCTDVLDATAVIKTGTCPFSFDKLNNYLTFNKFCLSLSPVGANCKFDVAARTRTNEQVVRSLYVIYEEGDNIVLVPRGSDYKDDDDK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: TRP33 TRP47 ARG50 GLU57 ASN59 ARG102 TYR103 ASP104 PRO105 TYR106 TYR107 MET109 B: ASP1 ILE2 GLN27 GLN89 THR91 ASP92 SER93 TRP94 THR96 E: LYS524 ARG525 SER526 GLY527 TYR528 GLY529 GLN530 ILE532 LEU570 TRP571 ARG630 THR631 ASN632 GLN634 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
