Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7170 |
| PDBID: | 5O8F |
| Chains: | L_C |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.20 |
| Reference: | 10.1038/nsmb.3484 |
| Antibody | |
| Antibody: | 25 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Chimaeric beta3-alpha5 type A γ-aminobutyric acid receptor (GABAA R) |
| Antigen mutation: | No |
| Durg Target: | P31644; P31644; |
Sequence information
Antibody
Chain: L
Mutation: NULL
| >5O8F_L|Chain G[auth L], H[auth M], I[auth N]|Nanobody Nb25|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCAASGHTFNYPIMGWFRQAPGKEREFVGAISWSGGSTSYADSVKDRFTISRDNAKNTVYLEMNNLKPEDTAVYYCAAKGRYSGGLYYPTNYDYWGQGTQVTVS |
Antigen
Chain: C
Mutation: NULL
| >5O8F_C|Chain A, B, C, D, E|Gamma-aminobutyric acid receptor subunit beta-3,Gamma-aminobutyric acid receptor subunit alpha-5,Gamma-aminobutyric acid receptor subunit alpha-5|Homo sapiens (9606) ETGQSVNDPGNMSFVKETVDKLLKGYDIRLRPDFGGPPVCVGMNIDIASIDMVSEVNMDYTLTMYFQQYWRDKRLAYSGIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGGDKAVTGVERIELPQFSIVEHRLVSRNVVFATGAYPRLSLSFRLKRNIGYFVIQTYLPCIMTVILSQVSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKSQPARAAKIDKMSRIVFPILFGTFNLVYWATYLNREPVIKGATSPKGTTETSQVAPA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: PHE29 PRO32 ILE33 ALA50 ILE51 SER52 TRP53 SER54 GLY56 SER57 THR58 SER59 TYR60 ALA61 ASP62 LYS99 ARG101 TYR102 GLY104 GLY105 LEU106 TYR107 C: ALA45 LYS173 THR176 VAL178 GLU179 ARG180 ILE181 GLU182 LEU183 PHE186 SER187 ILE188 VAL189 HIS191 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
