Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7164 |
| PDBID: | 5O03 |
| Chains: | D_B |
| Organism: | Norwalk virus, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.19 |
| Reference: | 10.1371/journal.ppat.1006636 |
| Antibody | |
| Antibody: | Nano-32 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Norovirus capsid protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >5O03_D|Chain C, D|Nanobody (VHH) Nano-32|Lama glama (9844) QVQLQESGGGLVQPGGSLRLSCAASGFTLGYYPIGWFRQAPGKGLEGVSCISGSGGSANYAASVKGRFTISRDNAKNTVYLQMNSLKPEDTAIYYCAADLSSLTTVQAMCVIPRPGFSAKAYDYWGLGTQVTVSS |
Antigen
Chain: B
Mutation: NULL
| >5O03_B|Chain A, B|Capsid protein|Norwalk virus (11983) SKPFTLPILTLGELTNSRFPLPIDVLYTNPNESAIVQCQNGRCTLDGELQGTTQLLPTGICAFRGKVTQQVQDEHRGTHWNMTVTNLNGTPFDPTEDVPAPLGTPDFSGQIYGVISQRNTNTVPGEGNLPANRAHEAVIATYSPKFTPKLGNIQFSTWETQDVSSGQPTKFTPVGLASVDANSHFDQWTLPSYSGALTLNMNLAPSVAPVFPGECLLFFRSFIPLKGGYGNPAIDCLMPQEWVQHLYQESAPSLSDVALVRYVNPETGRTLFEAKLHRNGFLTVARNSAGPVVAPTNGYFRFDSWVNQFYTLAPM |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: GLN1 VAL2 GLY26 PHE27 TYR32 LEU100 SER101 SER102 LEU103 LYS120 ALA121 TYR122 ASP123 TYR124 B: THR233 GLU236 ASP247 VAL248 PHE445 ASN454 ASN487 GLU489 ARG492 LEU494 PRO514 VAL515 VAL516 ALA517 PRO518 THR519 ASN520 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
