Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7158 |
| PDBID: | 5MZV |
| Chains: | D_A |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.80 |
| Reference: | 10.1016/j.immuni.2017.12.008 |
| Antibody | |
| Antibody: | 22E11 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-23 (IL-23) |
| Antigen mutation: | No |
| Durg Target: | P29460 |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >5MZV_D|Chain D|Nanobody 22E11|Lama glama (9844) MKYLLPTAAAGLLLLAAQPAMAEVQLVESGGGLVQAGGSLRLSCAASGRTFSWSAVGWFRQAPGKEREFVAAIRWSGGSPYYADSVKDRFTISRDNAKNTVYLQMNSLRPEDTAVYLCGETSLFPTSRGSHYDTWGQGTQVTVSSGSGWSHPQFEK |
Antigen
Chain: A
Mutation: NULL
| >5MZV_A|Chain A|Interleukin-12 subunit beta|Homo sapiens (9606) MCHQQLVISWFSLVFLASPLVAIWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: ARG27 THR28 PHE29 SER30 TRP31 GLU44 ARG52 SER57 PRO58 TYR59 LYS65 SER100 LEU101 PHE102 PRO103 THR104 SER105 ARG106 HIS109 ASP111 A: LEU35 TRP37 PRO39 ASP40 LEU62 ASP63 GLU67 LYS80 GLU81 PHE82 GLY83 ALA85 LYS106 GLU108 ASP115 LYS118 THR147 ILE148 ASN184 HIS216 LYS217 LEU218 LYS219 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
