Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 715 |
| PDBID: | 4EIG |
| Chains: | B_A |
| Organism: | Escherichia coli K-12, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1016/j.bbapap.2013.07.010 |
| Antibody | |
| Antibody: | CA1698 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Dihydrofolate reductase |
| Antigen mutation: | No |
| Durg Target: | P0ABQ4 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >4EIG_B|Chain B|CA1698 camel antibody fragment|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCKASGIIFSVYKMTWYRQAPGKERELVALITTNNNTMTVDSVKGRFTISRDNVQNTVYLEMNNLKPEDTAVYYCNANRGLAGPAYWGQGTQVTVSSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >4EIG_A|Chain A|Dihydrofolate reductase|Escherichia coli (83333) MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition B: VAL31 LYS33 THR35 TYR37 GLU44 ARG45 GLU46 LEU47 LEU50 THR52 THR53 ASN54 ASN56 THR57 MET58 VAL60 ASN96 ASN98 ARG99 GLY100 LEU101 ALA102 GLY103 PRO104 A: GLU17 ASN18 ALA19 MET20 PRO21 TRP22 ASN23 PRO25 ASP27 LEU28 PHE31 THR46 GLU48 SER49 ILE50 GLY51 ARG52 PRO53 LEU54 PRO55 ILE94 VAL119 ALA145 GLN146 SER148 HIS149 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
