Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7144 |
| PDBID: | 5LXA |
| Chains: | H_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1038/nature21714 |
| Antibody | |
| Antibody: | 5LX9 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Adiponectin receptor 2 (ADIPOR2) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: H
Mutation: NULL
| >5LXA_H|Chain B[auth H]|Anti-CD30 moab Ki-4 scFv|Mus musculus (10090) MGEVLLQQSGPELVKPGASVRITCKASGYTFTDFNMDWVKQSPGKSLEWIGDFNPNSGGSIYNQKFKDKATFTVDKSSSTAYMELRSLTFEDTAVYYCARETGTAWFAYWGQGTLVTVSAAGGGGSGGGGSGGGGSGGGGSDIQMTQSPASLSASVGETVTITCRASGNIHNFLAWYQQKQGKSPQVLVYNAKTLADGVPSRFSGSGSGTQYSLKINSLQPEDFGSYYCQQFWSTPYTFGGGTKLEINAAADDDDKAGWSHPQFEKGGGSGGGSGGGSWSHPQFEK |
Antigen
Chain: A
Mutation: NULL
| >5LXA_A|Chain A|Adiponectin receptor protein 2|Homo sapiens (9606) MDYKDDDDKENLYFQGGSEFEGRWRVIPHDVLPDWLKDNDFLLHGHRPPMPSFRACFKSIFRIHTETGNIWTHLLGCVFFLCLGIFYMFRPNISFVAPLQEKVVFGLFFLGAILCLSFSWLFHTVYCHSEGVSRLFSKLDYSGIALLIMGSFVPWLYYSFYCNPQPCFIYLIVICVLGIAAIIVSQWDMFATPQYRGVRAGVFLGLGLSGIIPTLHYVISEGFLKAATIGQIGWLMLMASLYITGAALYAARIPERFFPGKCDIWFHSHQLFHIFVVAGAFVHFHGVSNLQEFRFMIGGGCSEEDAL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR30 ASP31 PHE32 ASN33 ASP50 ASN52 ASN54 ILE59 ARG98 GLU99 THR100 GLY101 THR102 ALA103 TYR107 ILE141 HIS169 ASN170 PHE171 TYR188 ASN189 THR192 PHE230 TRP231 SER232 THR233 TYR235 A: PHE0 GLU100 GLY101 ARG102 TRP103 ARG104 VAL105 ILE106 PRO107 VAL110 PRO112 TRP114 HIS123 PRO127 PRO128 MET129 PRO130 SER131 ARG133 ALA134 LYS137 HIS207 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
