Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 714 |
| PDBID: | 4EDX |
| Chains: | HL_V |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.4161/mabs.28677 |
| Antibody | |
| Antibody: | 911 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Beta-nerve growth factor |
| Antigen mutation: | No |
| Durg Target: | P01138 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4EDX_H|Chain C[auth B], F[auth H]|heavy chain of Fab of murine anti-NGF|Mus musculus (10090) QVQLKESGPGLVAPSQSLSITCTVSGFSLIGYDINWVRQPPGKGLEWLGMIWGDGTTDYNSALKSRLSISKDNSKSQVFLKMNSLRTDDTATYSCARGGYYYGTSYYFDYWGQGTTLTVSSASTTPPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPR |
Light Chain: L
Mutation: NULL
| >4EDX_L|Chain B[auth A], E[auth L]|light chain of FAB of murine anti-NGF|Mus musculus (10090) DIQMTQTTSSLSASLGDRVTISCRASQDISNHLNWYQQKPDGTVKLLIYYISRFHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQSKTLPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: V
Mutation: NULL
| >4EDX_V|Chain A[auth V], D[auth W]|Beta-nerve growth factor|Homo sapiens (9606) SSSHPIFHRGEFSVCDSVSVWVGDKTTATDIKGKEVMVLGEVNINNSVFKQYFFETKCRDPNPVDSGCRGIDSKHWNSYCTTTHTFVKALTMDGKQAAWRFIRIDTACVCVLSRKAVRRA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition L: TYR49 SER52 ARG53 PHE54 HIS55 SER56 SER60 SER63 GLY64 H: ILE30 GLY31 TYR32 ASP33 TRP52 GLY53 ASP54 GLY55 THR56 TYR97 TYR98 TYR99 V: GLY10 GLU11 PHE12 SER13 SER17 VAL18 SER19 VAL20 TRP21 GLY23 ASP24 PHE49 LYS50 TYR52 PHE54 THR56 ARG59 THR106 ALA107 CYS108 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
