Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7123 |
| PDBID: | 5HYS |
| Chains: | EF_J |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1038/ncomms11610 |
| Antibody | |
| Antibody: | Omalizumab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Omalizumab(Approved) |
| Antigen | |
| Antigen: | IgE Fc |
| Antigen mutation: | Yes |
| Durg Target: | P01854 |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >5HYS_E|Chain A, C, E, G[auth H]|Epididymis luminal protein 214|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAVSGYSITSGYSWNWIRQAPGKGLEWVASITYDGSTNYNPSVKGRITISRDDSKNTFYLQMNSLRAEDTAVYYCARGSHYFGHWHFAVWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: F
Mutation: NULL
| >5HYS_F|Chain B, D, F, H[auth L]|Uncharacterized protein|Homo sapiens (9606) DIQLTQSPSSLSASVGDRVTITCRASQSVDYDGDSYMNWYQQKPGKAPKLLIYAASYLESGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSHEDPYTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: J
Mutation: C4A/G11C
| >5HYS_J|Chain I[auth G], J[auth I], K[auth J], L[auth K]|Ig epsilon chain C region|Homo sapiens (9606) ADPAADSNPRCVSAYLSRPSPFDLFIRKSPTITCLVVDLAPSKGTVNLTWSRASGKPVNHSTRKEEKQRNGTLTVTSTLPVGTRDWIEGETYQCRVTHPHLPRALMRSTTKTSGPRAAPEVYAFATPEWPGSRDKRTLACLIQNFMPEDISVQWLHNEVQLPDARHSTTQPRKTKGSGFFVFSRLEVTRAEWEQKDEFICRAVHEAASPSQTVQRAVSVNPGKAADDDDK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition F: ARG24 SER28 ASP30 SER71 GLY72 THR73 ASP74 J: PRO365 SER366 LYS367 GLY368 HIS424 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
