Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 711 |
| PDBID: | 4DAG |
| Chains: | HL_A |
| Organism: | human metapneumovirus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.39 |
| Reference: | 10.1038/nsmb.2250 |
| Antibody | |
| Antibody: | DS7 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Metapneumovirus Fusion glycoprotein F0 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4DAG_H|Chain B[auth H]|Neutralizing Antibody DS7 heavy chain|Homo sapiens (9606) EVQLLESGGGLVQPGGSRRLSCAASGFTVSSSYMSWVRQTPGKGLEWISVFYSGGTTYYADAVKGRFSISMDTSKNTLHLQMNSLRVEDTAIYYCARVLSRASGMPDAFDIWGPGTMVTVSSASTKGPSVFPLAPSSAATSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKV |
Light Chain: L
Mutation: NULL
| >4DAG_L|Chain C[auth L]|Neutralizing Antibody DS7 light chain|Homo sapiens (9606) ELALIQPASVSVSPGQTASITCSGDKLGDKYASWYQQKPGQSPVLVIYQDSERPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCQAWDSSTAVFGGGTTLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECSM |
Antigen
Chain: A
Mutation: NULL
| >4DAG_A|Chain A|Fusion glycoprotein F0|Human metapneumovirus (162145) GLKESYLEESCSTITEGYLSVLRTGWYTNVFTLEVGDVENLTCTDGPSLIKTELDLTKSALRELKTVSADQLAREEQIENPRQSRFVLGAIALGVATAAAVTAGIAIAKTIRLESEVNAIKGALKTTNEAVSTLGNGVRVLATAVRELKEFVSKNLTSAINKNKCDIADLKMAVSFSQFNRRFLNVVRQFSDNAGITPAISLDLMTDAELARAVSYMPTSAGQIKLMLENRAMVRRKGFGILIGVYGSSVIYMVQLPIFGVIDTPCWIIKAAPSCSEKDGNYACLLREDQGWYCKNAGSTVYYPNDKDCETRGDHVFCDTAAGINVAEQSRECNINISTTNYPCKVSTGRHPISMVALSPLGALVACYKGVSCSIGSNRVGIIKQLPKGCSYITNQDADTVTIDNTVYQLSKVEG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: SER31 TYR33 TYR52 SER53 GLY54 THR56 TYR58 SER100 ARG101 ALA102 SER103 GLY104 MET105 PRO106 L: LYS46 GLY48 ASP49 LYS50 TYR51 TRP110 SER112 SER113 A: GLY18 LEU19 LYS20 GLU21 SER22 TYR23 LEU24 GLU25 GLU26 ILE31 GLU33 GLY34 PRO282 TRP284 LYS312 ARG348 GLU349 ASN351 ILE352 ASN353 THR356 ASN358 LEU378 TYR409 THR411 GLN413 ASP414 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
