Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7102 |
| PDBID: | 5EII |
| Chains: | AB_I |
| Organism: | Homo sapiens, Saccharomyces cerevisiae |
| Method: | XRD |
| Resolution (Å): | 2.44 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | 5EII Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Histone chaperone ASF1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >5EII_A|Chain A, D[auth H]|Fab Heavy Chain|Homo sapiens (9606) EISEVQLVESGGGLVQPGGSLRLSCAASGFNISYSSIHWVRQAPGKGLEWVASISSYYGSTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARSRGQASWDYWWAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: B
Mutation: NULL
| >5EII_B|Chain B, F[auth L]|Fab Light Chain|Homo sapiens (9606) SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQSSDDPITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: I
Mutation: NULL
| >5EII_I|Chain C[auth G], E[auth I]|Histone chaperone ASF1|Saccharomyces cerevisiae (4932) MSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSILVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEEELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: TYR31 TYR54 TYR55 ARG100 GLY101 GLN102 ALA103 SER104 TRP105 TYR107 TRP109 B: SER28 VAL29 SER30 SER31 ALA32 SER50 ARG66 SER92 I: LYS10 LEU12 GLU23 GLU25 PHE28 LYS35 HIS36 ASP37 ASP58 ILE60 LEU61 VAL62 GLY63 PRO64 PRO66 ASN70 LYS71 PHE72 VAL73 PHE74 SER75 ASP77 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
