Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7085 |
| PDBID: | 5CWS |
| Chains: | HG_J |
| Organism: | Homo sapiens, Thermochaetoides thermophila DSM 1495 |
| Method: | XRD |
| Resolution (Å): | 3.77 |
| Reference: | 10.1126/science.aac9176 |
| Antibody | |
| Antibody: | sAB-158 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Nucleoporin NUP49 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5CWS_H|Chain B, H|sAB-158 Fab Heavy Chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYAEISEVQLVESGGGLVQPGGSLRLSCAASGFNVYSYSIHWVRQAPGKGLEWVASIYSYYGSTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARLTWRSYRGVHALDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTGGSHHHHHH |
Light Chain: G
Mutation: NULL
| >5CWS_G|Chain A, G|sAB-158 Fab Light Chain|Homo sapiens (9606) MKSLLPTAAAGLLLLAAQPAMASDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQSSSSLITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: J
Mutation: NULL
| >5CWS_J|Chain D, J|Nucleoporin NUP49|Chaetomium thermophilum (759272) MSEALQQEIAKIDEEIQKCIRDKEAVDAFLPAHGEQLAAIPTDVNFVTRKSEGAHNALSSDILAIDQLRELVKQDADNARLSFKAIDNLKLPMQYHQAGLWSKQMGGAGTAGASGASADADGQSNADLISYFSKTADEMEEMMKKFEKTITEIEAHLTGVEAHAMAMQNVAAQSRNAAQGGVDERVYELAAVLREFEESILKVAGVVGGVKEGVTELQLRDFMGHGS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition G: SER53 SER54 SER73 SER75 SER76 ARG89 H: TYR58 TRP127 ARG128 TYR130 ARG131 J: ALA275 GLU278 GLN279 ALA282 THR285 ASP286 PHE289 VAL290 LYS293 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
