Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7084 |
| PDBID: | 5CWS |
| Chains: | HG_K |
| Organism: | Homo sapiens, Thermochaetoides thermophila DSM 1495 |
| Method: | XRD |
| Resolution (Å): | 3.77 |
| Reference: | 10.1126/science.aac9176 |
| Antibody | |
| Antibody: | sAB-158 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Nucleoporin NUP57 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5CWS_H|Chain B, H|sAB-158 Fab Heavy Chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYAEISEVQLVESGGGLVQPGGSLRLSCAASGFNVYSYSIHWVRQAPGKGLEWVASIYSYYGSTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARLTWRSYRGVHALDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTGGSHHHHHH |
Light Chain: G
Mutation: NULL
| >5CWS_G|Chain A, G|sAB-158 Fab Light Chain|Homo sapiens (9606) MKSLLPTAAAGLLLLAAQPAMASDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQSSSSLITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: K
Mutation: NULL
| >5CWS_K|Chain E, K|Nucleoporin NUP57|Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (759272) MYQKPIPEQIKLIVDKWNPNHPNCAFKTYLYNKVDEHTVPLYGPGPNEDPKEWEEALQRKPAPNFIPVLCSGFPSIVARLMLQRRVITEFNNKLHQINASLDAILSRHDLDHTVRAFNARRRHAELSRRCLHLAARVQVLRNRGYALSGDEDELKQKLQQIDKTLNDPAQGSRLEELWSRLIVLRGYAEDLKDQINQAGITESDGLGEEIEAKAKKILEDYDKQLQHLKKQVEEAKKDFEEWEKQHN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition G: TYR72 SER76 LEU77 TYR78 SER79 GLY80 SER86 H: GLU27 VAL28 GLY52 PHE53 ASN54 SER57 TYR58 TYR80 THR100 SER101 ASN103 ARG124 TRP127 ASP137 TYR138 K: ARG153 THR157 ASN160 ASN161 LEU163 HIS164 ASN167 ALA168 ASP171 ALA172 LEU174 SER175 ARG176 ASP178 LEU179 LYS284 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
