Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7077 |
| PDBID: | 5C7X |
| Chains: | MN_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.95 |
| Reference: | 10.1080/19420862.2015.1099774 |
| Antibody | |
| Antibody: | MOR04357 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Granulocyte macrophage colony stimulating factor (GM-CSF) |
| Antigen mutation: | No |
| Durg Target: | P04141 |
Sequence information
Antibody
Heavy Chain: M
Mutation: NULL
| >5C7X_M|Chain C[auth H], E[auth M]|Immunglobulin G1 Fab fragment, heavy chain|Homo sapiens (9606) QVQLVESGGGLVQPGGSLRLSCAASGFTFSSYWMNWVRQAPGKGLEWVSGIENKYAGGATYYAASVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARGFGTDFWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSEFDYKDDDDKGAPHHHHHH |
Light Chain: N
Mutation: NULL
| >5C7X_N|Chain D[auth L], F[auth N]|Immunglobulin G1 Fab fragment, light chain|Homo sapiens (9606) DIELTQPPSVSVAPGQTARISCSGDSIGKKYAYWYQQKPGQAPVLVIYKKRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCSAWGDKGMVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEA |
Antigen
Chain: B
Mutation: NULL
| >5C7X_B|Chain A, B|Granulocyte-macrophage colony-stimulating factor|Homo sapiens (9606) APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| M: THR28 SER31 TYR32 TRP33 TRP47 TYR52 ALA53 TYR58 GLY95 PHE96 GLY97 ASP101 N: GLY29 LYS30 LYS31 TYR32 TYR34 LYS50 LYS51 ARG52 ASN66 SER67 GLY68 TRP91 GLY92 ASP93 LYS94 GLY95 B: GLN11 PRO12 TRP13 HIS15 VAL16 ASN17 ILE19 GLN20 GLU21 ARG23 ARG24 MET46 PHE47 LEU49 HIS83 LYS85 GLN86 HIS87 CYS88 PRO89 PRO90 ASP112 LEU114 LEU115 VAL116 ILE117 PRO118 PHE119 ASP120 CYS121 TRP122 GLU123 PRO124 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
