Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7075 |
| PDBID: | 5C1M |
| Chains: | B_A |
| Organism: | Mus musculus, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.07 |
| Reference: | 10.1038/nature14886 |
| Antibody | |
| Antibody: | 39 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | μ-opioid receptor (μOR) |
| Antigen mutation: | No |
| Durg Target: | P35372 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >5C1M_B|Chain B|Nanobody 39|Lama glama (9844) QVQLVESGGGLVRPGGSLRLSCVDSERTSYPMGWFRRAPGKEREFVASITWSGIDPTYADSVADRFTTSRDVANNTLYLQMNSLKHEDTAVYYCAARAPVGQSSSPYDYDYWGQGTQVTVSSAAA |
Antigen
Chain: A
Mutation: NULL
| >5C1M_A|Chain A|Mu-type opioid receptor|Mus musculus (10090) GSHSLCPQTGSPSMVTAITIMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALATSTLPFQSVNYLMGTWPFGNILCKIVISIDYYNMFTSIFTLCTMSVDRYIAVCHPVKALDFRTPRNAKIVNVCNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENLLKICVFIFAFIMPVLIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITRMVLVVVAVFIVCWTPIHIYVIIKALITIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCF |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ARG21 GLU28 ARG29 THR30 TYR32 ILE51 THR52 TRP53 SER54 GLY55 ILE56 ASP57 PRO58 THR59 TYR60 ASP62 ALA65 ASP66 THR69 THR70 SER71 ARG72 ASP73 VAL74 ALA75 ASN77 TYR80 GLN82 ASN84 PRO101 VAL102 A: THR97 LYS100 THR101 THR103 ARG165 ALA168 VAL169 PRO172 VAL173 ALA175 LEU176 ASP177 ARG179 THR180 PRO181 MET255 ARG258 LEU259 VAL262 ARG263 MET264 LEU265 SER266 GLY267 GLU270 LYS271 ASN274 LEU275 ILE278 ASP340 GLU341 ASN342 ARG345 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
