Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7067 |
| PDBID: | 5AAW |
| Chains: | H_I |
| Organism: | Mus musculus, Dengue virus |
| Method: | XRD |
| Resolution (Å): | 3.27 |
| Reference: | 10.1016/j.cell.2015.06.057 |
| Antibody | |
| Antibody: | 513 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Dengue virus (DENV) 4 envelope glycoprotein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: H
Mutation: NULL
| >5AAW_H|Chain A, B, D, F, H, K|SCFV513|MUS MUSCULUS (10090) QQVQLVQSGAEVKKPGASVKVSCKAGFNIKDVYMSWVRQAPEQGLEWMGRIDPENGDTKYDPKLQGRVTMTADTSTNTAYMELRSLRSDDTAVYYCARGWEGFAYWGQGTLVTVSSGGGGSGGGGSGGGGSDIVMTQSPASLAVSLGQRATISCRASENVDKYGNSFMHWYQQKPGQPPKLLIYRASELQWGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQRSNEVPWTFGQGTKLEIKRTVAHHHHHH |
Antigen
Chain: I
Mutation: NULL
| >5AAW_I|Chain C, E, G, I, J, L|DENGUE SEROTYPE 4 ENVELOPE PROTEIN DOMAIN 3|DENGUE VIRUS (12637) MRIKGMSYTMCSGKFSIDKEMAETQHGTTVVKVKYEGAGAPCKVPIEIRDVNKEKVVGRIISSTPLAENTNSVTNIELEPPFGDSYIVIGVGNSALTLHWFRKGSSIGKHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLN4 GLY26 PHE27 ASN28 LYS30 ASP31 VAL32 TYR33 ASP52 GLU54 ARG98 GLY99 TRP100 GLU101 ALA104 TYR105 LYS163 TYR164 GLY165 ASN166 PHE168 TYR185 ARG186 GLU189 LEU190 GLN191 TRP192 ASN228 GLU229 I: LYS305 PHE306 SER307 ILE308 ASP309 LYS310 GLU311 MET312 LYS323 LYS325 GLU327 GLY328 ALA329 THR361 ASN362 SER363 VAL364 ASN366 ASN384 SER385 LEU387 THR388 LEU389 HIS390 TRP391 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
