Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7045 |
| PDBID: | 4UIF |
| Chains: | KL_C |
| Organism: | dengue virus type 2, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 6.50 |
| Reference: | 10.1126/science.aaa8651 |
| Antibody | |
| Antibody: | 2D22 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Dengue virus (DENV) type 2 envelope glycoprotein |
| Antigen mutation: | No |
| Durg Target: | P27914; P12823; |
Sequence information
Antibody
Heavy Chain: K
Mutation: NULL
| >4UIF_K|Chain G, I, K|ANTIGEN-BINDING FRAGMENT OF HUMAN ANTIBODY 2D22 - HEAVY CHAIN|HOMO SAPIENS (9606) EVQLVESGAEVKKPGSSVKVSCKASGGTFNNYAISWVRQAPGQGLEWMGGIIPIFGGANYAQKFQGRVTITADRSTSTVYMELSGLRSEDTAVYYCARRPQSIFDWNFDLWGRGTLVTVSSAGTKGPS |
Light Chain: L
Mutation: NULL
| >4UIF_L|Chain H, J, L|ANTIGEN-BINDING FRAGMENT OF HUMAN ANTIBODY 2D22 - LIGHT CHAIN|HOMO SAPIENS (9606) QSVLTQPPSASGTPGQRVTISCSGSSSNVGSNYVYWYQQLPGTAPKLLIYRNNRRPSGVPDRFSGSKSGTSASLAISGLRSEDEADYYCATWDDSLSGLVFGGGTKLTVLGQPKA |
Antigen
Chain: C
Mutation: NULL
| >4UIF_C|Chain A, C, E|DENGUE VIRUS SEROTYPE 2 STRAIN PVP94 07 - ENVELOPE PROTEIN|DENGUE VIRUS 2 (11060) MRCIGISNRDFVEGVSGGSWVDIVLEHGSCVTTMAKNKPTLDFELIKTEAKHPATLRKYCVEAKLTNTTTASRCPTQGEPSLNEEQDKRFVCKHSMVDRGWGNGCGLFGKGGIVTCAMFTCKKNMEGKVVQPENLEYTIVITPHSGEENAVGNDTGKHGKEIKVTPQSSITEAELTGYGTVTMECSPRTGLDFNEMVLLQMENKAWLVHRQWFLDLPLPWLPGADTQGSNWIQKETLVTFKNPHAKKQDVVVLGSQEGAMHTALTGATEIQMSSGNLLFTGHLKCRLRMDKLQLKGMSYSMCTGKFKVVKEIAETQHGTIVIRVQYEGDGSPCKIPFEIMDLEKRHVLGRLITVNPIVTEKDSPVNIEAEPPFGDSYIIIGVEPGQLKLSWFKKGSSIGQMFETTMRGAKRMAILGDTAWDFGSLGGVFTSIGKALHQVFGAIYGAAFSGVSWTMKILIGVVITWIGMNSRSTSLSVSLVLVGVVTLYLGVMVQA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| K: PHE55 GLY56 GLY57 ASP73 SER75 ILE103 PHE104 C: ASN67 THR68 THR69 THR70 ALA71 SER72 ARG73 TRP101 GLY102 ASN103 GLY104 LYS247 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
